Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales)
Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales)
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微管蛋白和中心蛋白同源物在营养细胞中的定位以及 Ectocarpus siliculosus (Dillw.) Lyngb 的配子体发育。
DOI：
10.1111/j.1438-8677.1991.tb00201.x
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发表时间：
1991
影响因子：
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通讯作者：
M. Melkonian
中科院分区：
文献类型：
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作者：
C. Katsaros;G. Kreimer;M. Melkonian
用免疫荧光法研究了微管蛋白和中心素在海鞘营养细胞和配子发生过程中的分布。在间期细胞中，微管束集中在靠近核表面的中心粒区域。一些束包裹着细胞核，而另一些则以不同的方向穿过细胞质，有时到达细胞皮质。激光共聚焦扫描显微镜(CLSM)对连续光学切片的评价表明，核周微管束和“细胞质”微管束可能构成一个单一的复合体。在间期细胞中，中心蛋白定位为中心粒区域的单一亮点。在细胞分裂过程中，微管复合体和中心点发生复制和分离。在具有两个核的有丝分裂后细胞中，中心粒位于相对的细胞极，从中发出短的微管束，并部分包围细胞核。在配子发生过程中，营养细胞骨架逐渐转变为配子鞭毛器。
The distribution of tubulin and centrin in vegetative cells and during gametogenesis of Ectocarpus siliculosus was studied by immunofluorescence. In interphase cells bundles of microtubules are focused on the centriolar region near the nuclear surface. Some of the bundles ensheath the nucleus while others traverse the cytoplasm in various directions, sometimes reaching the cell cortex. Evaluation of serial optical sections by confocal laser scanning microscopy (CLSM) revealed that the perinuclear and “cytoplasmic” microtubule bundles presumably constitute a single complex. In interphase cells centrin is localized as a single bright spot in the centriolar region. In dividing cells duplication and separation of the microtubular complex and the centrin spot takes place. In post-mitotic cells with two nuclei, the centrioles are located at opposite cell poles, short microtubule bundles emanate from them and partially encompass the nucleus. During gametogenesis a gradual transformation of the vegetative cytoskeleton to the gametic flagellar apparatus occurs.
影响因子：
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作者：
Salisbury,JL;Baron,AT;Coling,DE;Martindale,VE;Sanders,MA
通讯作者：
Sanders,MA