Effect of human beta-defensin-3 on head and neck cancer cell migration using micro-fabricated cell islands.
Effect of human beta-defensin-3 on head and neck cancer cell migration using micro-fabricated cell islands.
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DOI：
10.1186/1758-3284-4-41
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发表时间：
2012-06-28
期刊：
影响因子：
--
通讯作者：
Jurevic R
中科院分区：
文献类型：
--
作者：
Wang K;Wang JH;Baskaran H;Wang R;Jurevic R
采用微加工和软光刻技术研究天然抗菌肽人β-防御素-3（hBD-3）对头颈癌细胞系体外迁移的影响。将TR 146癌细胞接种在具有微制造的威尔斯孔的培养皿中用于细胞迁移测定。共使用了54个不同形状和大小以及实验培养基类型的细胞岛。在6组培养基中分析细胞迁移实验：Dulbecco改良培养基（DMEM）;含有血管内皮生长因子（VEGF）的DMEM;通过转染克隆的pcDNA 3表达hBD-3的人胚肾细胞（HEK 239）的条件培养基（CM/hBD-3）; CM/hBD-3 + VEGF;来自未转染的HEK 239的条件培养基（CM/hBD-3 + VEGF）。（不表达hBD-3）作为对照组（CM）; CM + VEGF组。细胞岛为圆形或方形，大小不同（0.25 mm 2、0.125 mm 2和0.0625 mm 2）。在t = 0 h、3 h、6 h和24 h对细胞岛进行成像。结果表明，最初较小的癌细胞岛具有较高的迁移指数。圆形和方形细胞岛的MI无差异。除CM组和CM-hBD-3+ VEGF组外，各组终点MI均存在显著差异。VEGF增强癌细胞迁移。DMEM和VEGF的组合显示出对癌细胞迁移的这种现象的协同作用。含有hBD-3的条件培养基抑制癌细胞迁移。hBD-3抑制TR 146癌细胞迁移的VEGF增强。
To examine the effect of the natural antimicrobial peptide human β-defensin-3 (hBD-3), on the migration of a head and neck cancer cell line in vitro using microfabrication and soft-lithographic techniques. TR146 cancer cells were seeded in Petri dishes with microfabricated wells for cell migration assays. Total 54 cell islands were used of various shape and size and experimental media type. Cell migration assays were analyzed in six group media: Dulbecco’s modified medium (DMEM); DMEM with vascular endothelial growth factor (VEGF); Conditioned media of human embryonic kidney cells (HEK 239) expressing hBD-3 via transfected cloned pcDNA3 as CM/hBD-3; CM/hBD-3 + VEGF; conditioned medium from non-transfected HEK 239 (not expressing hBD-3) as control (CM); and the last group was CM + VEGF. Cell islands were circular or square and varied in size (0.25 mm2, 0.125 mm2, and 0.0625 mm2). Cell islands were imaged at t = 0 h, 3 h, 6 h, and 24 h. The results show cancer cell islands that originally were smaller had higher migration indices. There was no difference of MIs between circular and square cell islands. MIs at the end point were significantly different among the groups except between CM and CM-hBD-3+ VEGF. VEGF enhanced cancer cell migration. The combination of DMEM and VEGF showed a synergistic effect on this phenomenon of cancer cell migration. Conditioned medium with hBD-3 suppressed cancer cell migration. hBD-3 suppressed VEGF enhancement of TR146 cancer cell migration.
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