Utilization of nucleic acid amplification assays for the detection of respiratory viruses.
Utilization of nucleic acid amplification assays for the detection of respiratory viruses.
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DOI：
10.1016/j.cll.2009.07.008
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发表时间：
2009-12
影响因子：
1.7
通讯作者：
Kumar S
中科院分区：
文献类型：
--
作者：
Kehl SC;Kumar S
Viruses are major contributors to morbidity and mortality from acute respiratory infections in all age groups worldwide. Accurate identification of the etiologic agent of respiratory tract infections is important for proper patient management. Diagnosis can be problematic, because a range of potential pathogens can cause similar clinical symptoms. Nucleic acid amplification testing is emerging as the preferred method of diagnostic testing. Real-time technology and the ability to perform multiplex testing have facilitated this emergence. Commercial platforms for nucleic acid amplification testing of respiratory viruses include real-time polymerase chain reaction (PCR), nucleic acid sequence-based amplification, and loop-mediated isothermal amplification. Multiplex PCR with fluidic microarrays or DNA chips are the most recent diagnostic advance. These assays offer significant advantages in sensitivity over antigen detection methods and in most cases also over traditional culture methods. A limited number of assays, however, are commercially available, thus laboratory developed assays frequently are used. This article reviews the performance of commercially available assays and discusses issues relevant to the development of in-house assays.
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影响因子：
9.4
作者：
Habib-Bein, NF;Beckwith, WH;Landry, ML
通讯作者：
Landry, ML
影响因子：
9.4
作者：
Hu, AZ;Colella, M;Cheng, SM
通讯作者：
Cheng, SM
影响因子：
9.4
作者：
Kuypers, Jane;Wright, Nancy;Morrow, Rhoda
通讯作者：
Morrow, Rhoda
影响因子：
9.4
作者：
LeGoff, Jerome;Kara, Rachid;Lebon, Pierre
通讯作者：
Lebon, Pierre
DOI：
10.1056/nejmoa0804877
发表时间：
2009-02-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
Hall CB;Weinberg GA;Iwane MK;Blumkin AK;Edwards KM;Staat MA;Auinger P;Griffin MR;Poehling KA;Erdman D;Grijalva CG;Zhu Y;Szilagyi P
通讯作者：
Szilagyi P