Decorin modulates collagen matrix assembly and mineralization.
Decorin modulates collagen matrix assembly and mineralization.
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DOI：
10.1016/j.matbio.2008.11.003
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Yamauchi M
中科院分区：
文献类型：
--
作者：
Mochida Y;Parisuthiman D;Pornprasertsuk-Damrongsri S;Atsawasuwan P;Sricholpech M;Boskey AL;Yamauchi M
核心蛋白聚糖（DCN）是骨中主要的基质蛋白聚糖之一。为了研究DCN在基质矿化中的作用，对MC 3 T3-E1（MC）细胞培养物中DCN的表达以及表达较高（有义; S-DCN）或较低（反义; AS-DCN）水平DCN的MC衍生克隆的表型进行了表征。随着矿化结节的形成和扩大，DCN的表达显著降低。在S-DCN克隆中，体外基质矿化被抑制，而在AS-DCN克隆中，矿化被加速。在显微镜下，与对照组相比，S-DCN克隆中的胶原纤维较细，而AS-DCN克隆中的胶原纤维较粗且缺乏方向性。在超微结构水平上，S-DCN克隆中的胶原纤维明显变细，而AS-DCN克隆中的胶原纤维较大且形状不规则。傅里叶变换红外光谱分析结果表明，在矿化早期，AS-DCN培养物中矿物含量高于对照，但矿物结晶度低于对照。体内移植试验表明，S-DCN移植物中没有形成矿化基质，而在移植3周时，在AS-DCN移植物中很容易检测到矿化基质。AS-DCN移植物中骨样基质的面积在3周时显著大于对照组，但在5周时变得相当。AS-DCN移植物中的骨样基质表现出编织骨样非层状结构，而对照移植物中的层状骨样结构明显。这些结果表明，DCN通过调节胶原组装来调节基质矿化。
Decorin (DCN) is one of the major matrix proteoglycans in bone. To investigate the role of DCN in matrix mineralization, the expression of DCN in MC3T3-E1 (MC) cell cultures and the phenotypes of MC-derived clones expressing higher (sense; S-DCN) or lower (antisense; AS-DCN) levels of DCN were characterized. DCN expression was significantly decreased as the mineralized nodules were formed and expanded in vitro. In S-DCN clones, in vitro matrix mineralization was inhibited, whereas in AS-DCN clones, mineralization was accelerated. At the microscopic level, collagen fibers in S-DCN clones were thinner while those of AS-DCN clones were thicker and lacked directionality compared to the controls. At the ultrastructural level, the collagen fibrils in S-DCN clones were markedly thinner, whereas those of AS-DCN clones were larger and irregular in shape. The results from Fourier transform infrared spectroscopy analysis demonstrated that in AS-DCN cultures the mineral content was greater but the crystallinity of mineral was poorer than that of the controls at early stage of mineralization. The in vivo transplantation assay demonstrated that no mineralized matrices were formed in S-DCN transplants, whereas they were readily detected in AS-DCN transplants at 3 weeks of transplantation. The areas of bone-like matrices in AS-DCN transplants were significantly greater than the controls at 3 weeks but became comparable at 5 weeks. The bone-like matrices in AS-DCN transplants exhibited woven bone-like non-lamellar structure while the lamellar bone-like structure was evident in the control transplants. These results suggest that DCN regulates matrix mineralization by modulating collagen assembly.
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DOI：
10.1007/bf00266843
发表时间：
1989-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
DAYAN, D;HISS, Y;WOLMAN, M
通讯作者：
WOLMAN, M
影响因子：
4.2
作者：
Gadaleta, SJ;Paschalis, EP;Boskey, AL
通讯作者：
Boskey, AL
影响因子：
4.2
作者：
Boskey, AL;Spevak, L;Rosenberg, L
通讯作者：
Rosenberg, L
影响因子：
3.7
作者：
Beck, GR;Moran, E;Knecht, N
通讯作者：
Knecht, N
影响因子：
4.8
作者：
Keene, DR;San Antonio, JD;Iozzo, RV
通讯作者：
Iozzo, RV