Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR.
Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR.
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DOI：
10.1371/journal.pone.0242049
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Tsai CT
中科院分区：
文献类型：
--
作者：
Cortez FJ;Gebhart D;Robinson PV;Seftel D;Pourmandi N;Owyoung J;Bertozzi CR;Wilson DM;Maahs DM;Buckingham BA;Mills JR;Roforth MM;Pittock SJ;McKeon A;Page K;Wolf WA;Sanda S;Speake C;Greenbaum CJ;Tsai CT
胰岛自身抗体主要通过放射性测定来测量，以促进1型糖尿病的风险评估和诊断。然而，对放射性成分的依赖、大的样品体积和有限的通量使得放射性测定测试成本高且具有挑战性。我们开发了一个基于抗体检测的多重分析平台，用于GAD，IA-2和胰岛素自身抗体/抗体在1 μL血清中的样品保留测量。在7个不同的队列（n = 858）中开发并验证了该试验，其中大多数队列在分析前设盲。将ADAP测定法的测量值与放射性测定法进行比较，以确定相关性、一致性、一致性、临床灵敏度和特异性。ADAP与放射性分析的平均总体一致率在91%以上。平均临床敏感性和特异性分别为96%和97%。在IASP 2018研讨会上，ADAP在GAD和IA-2自身抗体的95%特异性（AS 95）评级和胰岛素自身抗体的顶级性能下实现了所有检测试剂盒的最高灵敏度。此外，在接受测试的39名T1 D患者亲属中，ADAP通过放射性测定正确识别了95%具有两种或更多自身抗体的高风险个体。总之，新的ADAP检测法可以可靠地检测1μL血清中的三种主要胰岛自身抗体/抗体，具有高灵敏度。这种新的检测方法可以提高儿科检测的依从性，并有助于更容易在社区范围内筛查胰岛自身抗体。
Islet autoantibodies are predominantly measured by radioassay to facilitate risk assessment and diagnosis of type 1 diabetes. However, the reliance on radioactive components, large sample volumes and limited throughput renders radioassay testing costly and challenging. We developed a multiplex analysis platform based on antibody detection by agglutination-PCR (ADAP) for the sample-sparing measurement of GAD, IA-2 and insulin autoantibodies/antibodies in 1 μL serum. The assay was developed and validated in 7 distinct cohorts (n = 858) with the majority of the cohorts blinded prior to analysis. Measurements from the ADAP assay were compared to radioassay to determine correlation, concordance, agreement, clinical sensitivity and specificity. The average overall agreement between ADAP and radioassay was above 91%. The average clinical sensitivity and specificity were 96% and 97%. In the IASP 2018 workshop, ADAP achieved the highest sensitivity of all assays tested at 95% specificity (AS95) rating for GAD and IA-2 autoantibodies and top-tier performance for insulin autoantibodies. Furthermore, ADAP correctly identified 95% high-risk individuals with two or more autoantibodies by radioassay amongst 39 relatives of T1D patients tested. In conclusion, the new ADAP assay can reliably detect the three cardinal islet autoantibodies/antibodies in 1μL serum with high sensitivity. This novel assay may improve pediatric testing compliance and facilitate easier community-wide screening for islet autoantibodies.
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影响因子：
9.3
作者：
Lampasona, Vito;Pittman, David L.;Hagopian, W. A.
通讯作者：
Hagopian, W. A.
影响因子：
16.2
作者：
Chiang JL;Kirkman MS;Laffel LM;Peters AL;Type 1 Diabetes Sourcebook Authors
通讯作者：
Type 1 Diabetes Sourcebook Authors
DOI：
10.1073/pnas.1711004115
发表时间：
2018-02-06
影响因子：
11.1
作者：
Tsai, Cheng-ting;Robinson, Peter V.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
影响因子：
18.2
作者：
Tsai CT;Robinson PV;Spencer CA;Bertozzi CR
通讯作者：
Bertozzi CR
高通量多重自身抗体检测同时筛查1型糖尿病和多种自身免疫性疾病
影响因子：
11.1
作者：
Gu, Yong;Zhao, Zhiyuan;Yu, Liping
通讯作者：
Yu, Liping