Comparison of aldosterone production among human adrenocortical cell lines.
Comparison of aldosterone production among human adrenocortical cell lines.
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DOI：
10.1055/s-0031-1298019
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发表时间：
2012-03
期刊：
影响因子：
--
通讯作者：
Rainey WE
中科院分区：
文献类型：
--
作者：
Wang T;Rowland JG;Parmar J;Nesterova M;Seki T;Rainey WE
几个人类肾上腺皮质细胞系已被用作生产醛固酮的模型系统。然而，这些细胞系之间并没有进行直接的比较。比较了人肾上腺细胞系SW13、CAR47、NCI-H295及其亚株和亚克隆的醛固酮产量和醛固酮合成酶(CYP11B2)的表达。孵育48h后收集培养液，测定醛固酮分泌量，并将数据归一化为细胞蛋白量。提取RNA用于基因芯片分析和定量RT-PCR。在血管紧张素II(Ang II)刺激方面，进一步测试了具有最高醛固酮产量的细胞系。在SW13和CAR47细胞中均未检测到醛固酮和CYP11B2的转录本。NCI-H295、H295A、H295R-S1、H295R-S2、H295R-S3、HAC13、HAC15和HAC50的醛固酮产量分别为119、1、6、826、18、139、412和1334(pmol/mg蛋白/48h)。H295A和H295R-S1的细胞色素P11B2的表达低于常用的H295R-S3细胞，而NCI-H295、H295R-S2、HAC13、HAC15和HAC50的表达分别是H295R-S3细胞的24、14、3、10和35倍。经血管紧张素转换酶II处理后，NCI-H295、H295R-S2、HAC13、HAC15和HAC50的醛固酮产量显著高于基础水平(p<；0.05)。对现有的人肾上腺细胞系的比较表明，H295R-S2和克隆细胞系HAC13、HAC15和HAC50产生最高水平的醛固酮，并对Ang II反应良好。
Several human adrenocortical cell lines have been used as model systems for aldosterone production. However, these cell lines have not been directly compared with each other. Human adrenal cell lines SW13, CAR47, the NCI-H295 and its sub-strains and sub-clones were compared with regard to aldosterone production and aldosterone synthase (CYP11B2) expression. Culture media was collected 48 h after incubation, aldosterone secretion was measured and the data were normalized to the amount of cell protein. RNA was isolated for microarray analysis and quantitative RT-PCR (qPCR). The cell lines with the highest aldosterone production were further tested with regard to angiotensin II (Ang II) stimulation. Neither aldosterone nor CYP11B2 transcript were detected in SW13 or CAR47 cells. The aldosterone production by the NCI-H295, H295A, H295R-S1, H295R-S2, H295R-S3, HAC13, HAC15 and HAC50 were 119, 1, 6, 826, 18, 139, 412, and 1334 (pmol/mg protein/48h), respectively. H295A and H295R-S1 expressed less CYP11B2 than the commonly used H295R-S3 cells; while NCI-H295, H295R-S2, HAC13, HAC15 and HAC50 expressed 24, 14, 3, 10 and 35 fold higher CYP11B2 compared with the H295R-S3 cells. When treated with Ang II, NCI-H295, H295R-S2, HAC13, HAC15 and HAC50 showed significantly higher aldosterone production than the basal level (p<0.05). A comparison of the available human adrenal cell lines indicates that the H295R-S2 and the clonal cell lines, HAC13, HAC15 and HAC50 produced the highest levels of aldosterone and responded well to Ang II.
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影响因子：
4.1
作者：
Nogueira EF;Bollag WB;Rainey WE
通讯作者：
Rainey WE
影响因子：
--
作者：
CLARK, BJ;SOO, SC;STOCCO, DM
通讯作者：
STOCCO, DM
影响因子：
5.8
作者：
Parmar, Jeniel;Key, Rebecca E.;Rainey, William E.
通讯作者：
Rainey, William E.
影响因子：
5.8
作者：
Almeida, Madson Q.;Barisson Villares Fragoso, Maria Candida;Latronico, Ana Claudia
通讯作者：
Latronico, Ana Claudia
影响因子：
4.1
作者：
RAINEY, WE;BIRD, IM;MASON, JI
通讯作者：
MASON, JI