Sequential expression of maternally inherited phosphoglycerate kinase-1 in the early mouse embryo.
Sequential expression of maternally inherited phosphoglycerate kinase-1 in the early mouse embryo.
复制标题
母系遗传的磷酸甘油酸激酶-1 在早期小鼠胚胎中的顺序表达。
DOI：
10.1111/j.1432-0436.1987.tb00148.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
W. Krietsch
中科院分区：
文献类型：
--
作者：
R. Fundele;K. Illmensee;E. Jägerbauer;M. Fehlau;W. Krietsch
Enzyme activities of X-linked phosphoglycerate kinase (PGK-1) and autosomal glucose phosphate isomerase (GPI-1) were determined in intact mouse blastocysts and isolated inner cell masses (ICMs). Blastocysts were recovered from the uterus on day 4 of gestation and cultured overnight in vitro. ICMs were isolated by treatment with calcium ionophore A23187. On day 4, approximately 35% of the total activity of both PGK-1 and GPI-1 was located in the ICM. After overnight culture, the PGK-1 activity of the whole blastocyst nearly doubled, due to the activation of only the maternally derived gene coding for PGK-1. In the ICM, however, a pronounced decrease of PGK-1 activity was measured: only 10% of the total PGK-1 activity was measured in the ICM on day 5. In contrast to PGK-1, GPI-1 activity of the intact blastocyst remained stable from day 4 to day 5. In the ICM, the GPI-1 activity did decline, but to a lesser extent than PGK-1 activity: 20% of total GPI-1 activity was found in the ICM on day 5. These results, when compared with the data of Handyside and Hunter, suggest that the decline in GPI-1 activity in the ICM is due to a change in the ratio of trophectoderm (TE) to ICM cells. The greater reduction of PGK-1 activity in the ICM cannot, however, be explained solely by this mechanism. To explain the observed additional decrease, we postulate that Pgk-1 is not activated in the ICM prior to day 6. This implies that on day 4 maternal Pgk-1 is activated in the TE exclusively.
植入前小鼠胚胎中父本和母本 Gpi-1 表达的开始。
影响因子：
2.7
作者：
Gilbert,SF;Solter,D
通讯作者：
Solter,D