Onset of paternal and maternal Gpi-1 expression in preimplantation mouse embryos.

Onset of paternal and maternal Gpi-1 expression in preimplantation mouse embryos.
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植入前小鼠胚胎中父本和母本 Gpi-1 表达的开始。

DOI:
10.1016/0012-1606(85)90477-4
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发表时间:
1985
影响因子:
2.7
通讯作者:
Solter,D
Solter,D
中科院分区:
生物学3区
文献类型:
--
作者:
Gilbert,SF;Solter,D

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研究了葡萄糖磷酸异构酶基因GPI-1在两种不同等位基因的小鼠之间移植产生的小鼠胚胎中的初始激活。胚胎细胞核编码的蛋白质同工酶在胚胎发育的第4天首次被检测到,母本和父本基因被同时激活。比较这些核移植胚胎和这两个品系的F1胚胎产生的同工酶表明,当这些基因第一次被激活时,GPI-1的卵母细胞mRNA是缺失的。因此,GPI-1的存在来自于新转录的由母本和父本基因共同贡献的mRNA。母体细胞质GPI-1酶的相对比例从第3天到第6天下降,因此在第6天几乎没有检测到卵母细胞GPI-1。
The initial activation of the glucose phosphate isomerase gene,Gpi-1, was studied in mouse embryos produced by transplanting pronuclei between two strains of mice differing in alleles for this enzyme. Protein isozymes encoded by the embryonic cell nuclei were first detected on Day 4 of embryogenesis, and the maternal and paternal genes are seen to be activated simultaneously. Comparison of isozymes produced by these nuclear-transfer embryos and by F1embryos from these two strains suggests the absence of oocyte mRNA for GPI-1 at the time when these genes are first activated. Thus, the GPI-1 present is derived from newly transcribed mRNA contributed by both maternal and paternal genes. The relative proportion of maternal cytoplasmic GPI-1 enzyme declines from Day 3 to Day 6, such that on Day 6, almost no oocyte GPI-1 is detected.
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DOI: 10.1111/j.1432-0436.1982.tb01276.x
发表时间: 1982
期刊: Differentiation; research in biological diversity
影响因子: --
作者:
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DOI: --
发表时间: 1981
期刊:
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DOI: --
发表时间: 1971
影响因子: 2.7
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DOI: 10.1126/science.6857250
发表时间: 1983-01-01
期刊: SCIENCE
影响因子: 56.9
作者:
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通讯作者: SOLTER, D