An illustration of the potential for mapping MRI/MRS parameters with genetic over-expression profiles in human prostate cancer.
An illustration of the potential for mapping MRI/MRS parameters with genetic over-expression profiles in human prostate cancer.
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DOI：
10.1007/s10334-008-0133-3
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发表时间：
2008-11
影响因子：
2.3
通讯作者：
Gaston, Sandra M.
中科院分区：
文献类型：
--
作者：
Lenkinski, Robert E.;Bloch, B. Nicolas;Liu, Fangbing;Frangioni, John V.;Perner, Sven;Rubin, Mark A.;Genega, Elizabeth M.;Rofsky, Neil M.;Gaston, Sandra M.
磁共振成像（MRI）和MR波谱可以探测前列腺癌的各种生理（例如血管渗透性）和代谢特征。然而，人们对前列腺癌中观察到的光谱和成像特征的基因表达变化知之甚少。肿瘤诱导的血管通透性和血管生成的变化被认为有助于前列腺癌的动态对比度增强（DCE）MRI图像的模式，即使肿瘤血管生成的遗传基础是复杂的，这些DCEMRI功能的具体机制尚未确定。为了确定在人类前列腺癌中对应于MRS和DCEMRI模式的基因表达的变化，我们已经利用组织打印微剥离技术来生成对应于术前MRI/MRS研究的根治性前列腺切除术标本的“整体安装”分子图。这些分子图谱包括来自Affyssin基因芯片微阵列和定量逆转录酶PCR（qrt-PCR）分析以及免疫组织化学研究的RNA表达谱。在前列腺癌患者中使用这些方法，我们发现胆碱激酶a在大多数原发性肿瘤中过度表达。我们还观察到神经肽Y（NPY），一种新发现的血管生成因子，在DCEMRI阳性前列腺癌的一个子集的过度表达。这些研究为建立MRI/MRS参数作为人类前列腺癌的有效生物标志物奠定了基础。
Magnetic resonance imaging (MRI) and MR spectroscopy can probe a variety of physiological (e.g. blood vessel permeability) and metabolic characteristics of prostate cancer. However, little is known about the changes in gene expression that underlie the spectral and imaging features observed in prostate cancer. Tumor induced changes in vascular permeability and angiogenesis are thought to contribute to patterns of dynamic contrast enhanced (DCE) MRI images of prostate cancer even though the genetic basis of tumor vasculogenesis is complex and the specific mechanisms underlying these DCEMRI features have not yet been determined. In order to identify the changes in gene expression that correspond to MRS and DCEMRI patterns in human prostate cancers, we have utilized tissue print micropeel techniques to generate “whole mount” molecular maps of radical prostatectomy specimens that correspond to pre-surgical MRI/MRS studies. These molecular maps include RNA expression profiles from both Affymetrix GeneChip microarrays and quantitative reverse transcriptase PCR (qrt-PCR) analysis, as well as immunohistochemical studies. Using these methods on patients with prostate cancer, we found robust over-expression of choline kinase a in the majority of primary tumors. We also observed overexpression of neuropeptide Y (NPY), a newly identified angiogenic factor, in a subset of DCEMRI positive prostate cancers. These studies set the stage for establishing MRI/MRS parameters as validated biomarkers for human prostate cancer.
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影响因子：
3.3
作者：
Alonzi, Roberto;Padhani, Anwar R.;Allen, Clare
通讯作者：
Allen, Clare
影响因子：
--
作者：
Andriole, Gerald;Djavan, Bob;Schroder, Fritz
通讯作者：
Schroder, Fritz
影响因子：
4.8
作者：
Chaib, H;Cockrell, EK;Macoska, JA
通讯作者：
Macoska, JA
影响因子：
2.1
作者：
Efstathiou, Jason A.;Chen, Ming-Hui;D'Amico, Anthony V.
通讯作者：
D'Amico, Anthony V.
影响因子：
6.6
作者：
Han, M;Partin, AW;Walsh, PC
通讯作者：
Walsh, PC