Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses.
Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses.
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纤维噬菌体SIO-2的基因组和功能分析揭示了对海洋急救病毒的生态学和进化的新见解。
DOI：
10.1111/j.1462-2920.2011.02685.x
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发表时间：
2012-08
影响因子：
5.1
通讯作者：
Azam F
中科院分区：
文献类型：
--
作者：
Baudoux AC;Hendrix RW;Lander GC;Bailly X;Podell S;Paillard C;Johnson JE;Potter CS;Carragher B;Azam F
我们报告了一种新的海洋虹膜病毒，噬菌体Vibrio SIO-2的基因组和功能分析。该噬菌体可以裂解具有重要生态意义的相关弧菌物种，包括广泛拮抗的弧菌SWAT3以及Harveyi分支的著名成员（V. Harveyi ATTC BAA-1116和V. campbelllii ATCC 25920）。Vibrio噬菌体SIO-2具有80,598 bp的环状排列基因组，显示出不同寻常的特征。这个基因组比大多数已知的虹膜病毒的基因组大，并且在数据库中116个预测的蛋白质中只有38个具有同源物。另一种差异体现在核心基因的起源上，其中大多数与跨越广泛门的不相关病毒和细菌有着强烈的相似性。这些核心基因的排列顺序与大多数噬菌体相同，但它们不同寻常地间隔在两个地方，插入的DNA包含高密度的非特征基因。这些DNA插入物的获得与SIO-2衣壳的形态变化有关，SIO-2衣壳组装成一个大（80 nm）的壳，具有新颖的T=12对称性。这些非典型的结构特征赋予了SIO-2对各种物理、化学和环境因素的显著稳定性。鉴于这种高水平的功能和基因组新颖性，SIO-2成为生态和进化研究中相当感兴趣的模型。
We report on a genomic and functional analysis of a novel marine siphovirus, the Vibrio phage SIO-2. This phage is lytic for related Vibrio species of great ecological interest including the broadly antagonistic bacterium Vibrio sp. SWAT3 as well as notable members of the Harveyi clade (V. harveyi ATTC BAA-1116 and V. campbellii ATCC 25920). Vibrio phage SIO-2 has a circularly permuted genome of 80,598 bp, which displays unusual features. This genome is larger than that of most known siphoviruses and only 38 of the 116 predicted proteins had homologues in databases. Another divergence is manifest by the origin of core genes, most of which share robust similarities with unrelated viruses and bacteria spanning a wide range of phyla. These core genes are arranged in the same order as in most bacteriophages but they are unusually interspaced at two places with insertions of DNA comprising a high density of uncharacterized genes. The acquisition of these DNA inserts is associated with morphological variation of SIO-2 capsid, which assembles as a large (80 nm) shell with a novel T=12 symmetry. These atypical structural features confer on SIO-2 a remarkable stability to a variety of physical, chemical and environmental factors. Given this high level of functional and genomic novelty, SIO-2 emerges as a model of considerable interest in ecological and evolutionary studies.
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影响因子：
3.7
作者：
HENDRIX, RW;CASJENS, SR
通讯作者：
CASJENS, SR
影响因子：
3.7
作者：
Baudoux, AC;Brussaard, CPD
通讯作者：
Brussaard, CPD
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
2.9
作者：
HARRISON, PJ;WATERS, RE;TAYLOR, FJR
通讯作者：
TAYLOR, FJR
影响因子：
2.4
作者：
Austin, B.;Zhang, X-H.
通讯作者：
Zhang, X-H.