deepTools: a flexible platform for exploring deep-sequencing data.

deepTools: a flexible platform for exploring deep-sequencing data.
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DOI:
10.1093/nar/gku365
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发表时间:
2014-07
影响因子:
14.9
通讯作者:
Manke T
Manke T
中科院分区:
生物学2区
文献类型:
--
作者:
Ramírez F;Dündar F;Diehl S;Grüning BA;Manke T

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我们提出了一个基于银河系的Web服务器处理和可视化深度序列数据。Web服务器的核心功能包括一套新开发的工具,称为deepTools,使用户能够在标准化环境中探索其测序实验的结果。用户可以上传预处理的文件,其中包含标准格式的连续数据,并以直接但高度可定制的方式生成热图和摘要图。此外,我们还提供了几种工具,用于分析包含比对读数的文件,并能够高效且可重现地生成标准化覆盖率文件。作为一个模块化和开源的平台,deepTools可以很容易地扩展和定制,以满足未来的需求和发展。deepTools网络服务器可在http://deeptools.ie-freiburg.mpg.de上免费获得,并附有大量文档和教程,旨在传达深度测序数据分析的原则。无需注册即可使用Web服务器。deepTools可以在本地独立安装,也可以作为Galaxy的一部分安装。
We present a Galaxy based web server for processing and visualizing deeply sequenced data. The web server's core functionality consists of a suite of newly developed tools, called deepTools, that enable users with little bioinformatic background to explore the results of their sequencing experiments in a standardized setting. Users can upload pre-processed files with continuous data in standard formats and generate heatmaps and summary plots in a straight-forward, yet highly customizable manner. In addition, we offer several tools for the analysis of files containing aligned reads and enable efficient and reproducible generation of normalized coverage files. As a modular and open-source platform, deepTools can easily be expanded and customized to future demands and developments. The deepTools webserver is freely available at http://deeptools.ie-freiburg.mpg.de and is accompanied by extensive documentation and tutorials aimed at conveying the principles of deep-sequencing data analysis. The web server can be used without registration. deepTools can be installed locally either stand-alone or as part of Galaxy.
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发表时间: 2010
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