Comprehensive analysis of KLF2 as a prognostic biomarker associated with fibrosis and immune infiltration in advanced hepatocellular carcinoma.
Comprehensive analysis of KLF2 as a prognostic biomarker associated with fibrosis and immune infiltration in advanced hepatocellular carcinoma.
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DOI：
10.1186/s12859-023-05391-0
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发表时间：
2023-06-29
影响因子：
3
通讯作者：
Xiang, Zuo-Lin
中科院分区：
文献类型：
--
作者：
Chen, Xue-Qin;Ma, Jie;Xu, Di;Xiang, Zuo-Lin
Most Hepatocellular carcinoma (HCC) patients are in advanced or metastatic stage at the time of diagnosis. Prognosis for advanced HCC patients is dismal. This study was based on our previous microarray results, and aimed to explore the promising diagnostic and prognostic markers for advanced HCC by focusing on the important function of KLF2. The Cancer Genome Atlas (TCGA), Cancer Genome Consortium database (ICGC), and the Gene Expression Comprehensive Database (GEO) provided the raw data of this study research. The cBioPortal platform, CeDR Atlas platform, and the Human Protein Atlas (HPA) website were applied to analyze the mutational landscape and single-cell sequencing data of KLF2. Basing on the results of single-cell sequencing analyses, we further explored the molecular mechanism of KLF2 regulation in the fibrosis and immune infiltration of HCC. Decreased KLF2 expression was discovered to be mainly regulated by hypermethylation, and indicated a poor prognosis of HCC. Single-cell level expression analyses revealed KLF2 was highly expressed in immune cells and fibroblasts. The function enrichment analysis of KLF2 targets indicated the crucial association between KLF2 and tumor matrix. 33-genes related with cancer associated fibroblasts (CAFs) were collected to identify the significant association of KLF2 with fibrosis. And SPP1 was validated as a promising prognostic and diagnostic marker for advanced HCC patients. CXCR6 CD8+ T cells were noted as a predominant proportion in the immune microenvironment, and T cell receptor CD3D was discovered to be a potential therapeutic biomarker for HCC immunotherapy. This study identified that KLF2 is an important factor promoting HCC progression by affecting the fibrosis and immune infiltration, highlighting its great potential as a novel prognostic biomarker for advanced HCC. The online version contains supplementary material available at 10.1186/s12859-023-05391-0.
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影响因子：
29.4
作者：
Kim CK;He P;Bialkowska AB;Yang VW
通讯作者：
Yang VW
影响因子：
1.2
作者：
Aino, Hajime;Sumie, Shuji;Sata, Michio
通讯作者：
Sata, Michio
影响因子：
28.5
作者：
Huang MD;Chen WM;Qi FZ;Xia R;Sun M;Xu TP;Yin L;Zhang EB;De W;Shu YQ
通讯作者：
Shu YQ
DOI：
10.1146/annurev-pathol-052016-100322
发表时间：
2017-01-24
期刊：
Annual review of pathology
影响因子：
--
作者：
Affo S;Yu LX;Schwabe RF
通讯作者：
Schwabe RF
影响因子：
64.8
作者：
Engreitz JM;Haines JE;Perez EM;Munson G;Chen J;Kane M;McDonel PE;Guttman M;Lander ES
通讯作者：
Lander ES