Integration and transcription of human papillomavirus type 16 and 18 sequences in cell lines derived from cervical carcinomas.
Integration and transcription of human papillomavirus type 16 and 18 sequences in cell lines derived from cervical carcinomas.
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人乳头瘤病毒 16 型和 18 型序列在宫颈癌细胞系中的整合和转录。
DOI：
10.1099/0022-1317-68-2-583
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
B. Simizu
中科院分区：
文献类型：
--
作者：
H. Shirasawa;Y. Tomita;S. Sekiya;H. Takamizawa;B. Simizu
对5种来源于日本宫颈癌患者的细胞系SKG-I、SKG-II、SKG-IIIb、QG-U和QG-H进行了人乳头瘤病毒（HPV）DNA和病毒mRNA表达的检测。HPV 16型DNA与SKG-IIb、QG-U和QG-H细胞DNA共价连接，HPV 18型DNA与SKG-I和SKG-II细胞DNA共价连接。尽管HPV基因组的不同区域整合在这些细胞系中，但非编码区和早期区域（包括E6和E7开放阅读框（ORF））在所有细胞系中是保守的。通过用单切限制性内切酶消化DNA，在QG-H细胞中发现了HPV 16的完整基因组。其他早期区域ORF E1、E2、E4和E5被侧翼宿主细胞DNA中断，表明整合到宿主细胞DNA中优先发生在该区域。HPV特异性mRNA种类在所有五个细胞系中检测到。在含有HPV 16基因组的三个细胞系中，mRNA与基因组的早期区域杂交，覆盖整个E6和E7 ORF以及E1 ORF的一小部分，尽管这些细胞系中主要mRNA的量和大小不同。这些mRNA不与含有L1和L2 ORF的HPV基因组的晚期区域杂交。在SKG-II、SKG-IIIb和QG-H细胞中，我们还检测到c-myc和c-Ha-ras mRNA的表达水平约为正常细胞的9倍。
Five cell lines, SKG-I, SKG-II, SKG-IIIb, QG-U and QG-H derived from cervical carcinomas of Japanese patients, were examined for the presence of human papillomavirus (HPV) DNA and the expression of viral mRNA. The DNA of HPV type 16 was shown to be linked covalently with SKG-IIb, QG-U and QG-H cell DNA, and HPV 18 DNA with SKG-I and SKG-II cell DNA. Although different regions of the HPV genome were integrated in these cell lines, the non-coding region and an early region including the E6 and E7 open reading frames (ORFs) were conserved in all cell lines. The complete genome of HPV 16 was found in QG-H cells by digestion of the DNA with a single-cut restriction enzyme. The other early region ORFs E1, E2, E4 and E5 were interrupted by flanking host cell DNA, suggesting that the integration into host cell DNA occurs preferentially in this region. HPV-specific mRNA species were detected in all five cell lines. In the three cell lines containing the HPV 16 genome, mRNAs hybridized with the early region of the genome, covering the entire E6 and E7 ORFs and a minor part of the E1 ORF, although the amount and size of the major mRNAs varied in these cell lines. These mRNAs did not hybridize with the late region of the HPV genome containing the L1 and L2 ORFs. In SKG-II, SKG-IIIb and QG-H cells we also detected c-myc and c-Ha-ras mRNA expression at about nine times the level of that in normal cells.
DOI：
10.1073/pnas.83.13.4680
发表时间：
1986-07-01
影响因子：
11.1
作者：
SMOTKIN, D;WETTSTEIN, FO
通讯作者：
WETTSTEIN, FO
新建立的宫颈癌细胞系 (SKG-III)，具有 Regan 同工酶、人绒毛膜促性腺激素 β 亚基和妊娠特异性 β 1 糖蛋白表型。
影响因子：
11.2
作者：
Nozawa,S;Udagawa,Y;Ohta,H;Kurihara,S;Fishman,WH
通讯作者：
Fishman,WH