Mechanism of impaired skin collagen maturity in riboflavin or pyridoxine deficiency
Mechanism of impaired skin collagen maturity in riboflavin or pyridoxine deficiency
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核黄素或吡哆醇缺乏导致皮肤胶原成熟度受损的机制
DOI：
10.1007/bf02702670
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发表时间：
1990
影响因子：
2.9
通讯作者：
M. Bamji
中科院分区：
文献类型：
--
作者：
R. Lakshmi;A. Lakshmi;M. Bamji
为了阐明吡哆醇或核黄素缺乏大鼠皮肤胶原成熟受损的生化基础，测试了以下两种可能的机制：（i）皮肤赖氨酰氧化酶活性降低（EC 1·4·3·13），其启动胶原的交联，和（ii）假定的同型半胱氨酸水平升高，导致赖氨酸的中和吡哆醇缺乏时，皮肤赖氨酰氧化酶活性不受影响，提示磷酸吡哆醛可能不是其辅因子。在核黄素缺乏症，赖氨酰氧化酶活性没有改变，在新再生的大鼠皮肤，但略有减少，观察到在皮肤的18天大的幼鼠。这可能与体重不足有关，而不是缺乏本身。再生皮肤的纯化盐溶性胶原蛋白的醛含量在两种缺陷中均显着降低。在这两种缺陷中观察到皮肤同型半胱氨酸浓度增加2至4倍。结果表明，皮肤同型半胱氨酸水平的增加可能是负责核黄素或吡哆醇缺乏时皮肤胶原成熟受损。
To elucidate the biochemical basis of impaired skin collagen maturity in pyridoxine-or riboflavin-deficient rats the following two mechanistic possibilities were tested: (i) Reduction in the activity of skin lysyl oxidase (EC 1·4·3·13) which initiates the cross-linking of collagen and (ii) putative rise in homocysteine level leading to neutralization of allysine (α-aminoadipic acid δ-5-semialdehyde)or hydroxyallysine (hydroxy α-aminoadepic acid (δ-semialdehyde) in collagen by the formation of thiazine complexes.Skin lysyl oxidase activity was not affected in pyridoxine deficiency suggesting that pyridoxal phosphate may not be its cofactor. In riboflavin deficiency, lysyl oxidase activity was not altered in the newly regenerated rat skin but a slight reduction was observed in the skin of 18-day-old rat pups. This could be related to the body weight deficit rather than deficiencyper se. Aldehyde content of purified salt soluble collagen of regenerated skin was significantly reduced in both the deficiencies. A 2 to 4-fold increase in the concentration of skin homocysteine was observed in both the deficiencies. The results suggest that increase in skin homocysteine level may be responsible for the impaired skin collagen maturity in riboflavin or pyridoxine deficiency.
通过吸收和共振拉曼光谱证明吡咯并喹啉醌作为赖氨酰氧化酶中的羰基辅因子。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Williamson,PR;Moog,RS;Dooley,DM;Kagan,HM
通讯作者：
Kagan,HM
开发赖氨酰氧化酶的过氧化物酶偶联荧光测定法。
影响因子：
2.9
作者：
Trackman,PC;Zoski,CG;Kagan,HM
通讯作者：
Kagan,HM