Increased prevalence of seropositivity for non-gastric Helicobacter species in patients with autoimmune liver disease.
Increased prevalence of seropositivity for non-gastric Helicobacter species in patients with autoimmune liver disease.
复制标题
自身免疫性肝病患者中非胃螺杆菌血清阳性率增加。
DOI：
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发表时间：
2003
影响因子：
3
通讯作者：
Torkel Wadström
中科院分区：
文献类型：
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作者：
I. Nilsson;Iryna Kornilovs’ka;Stefan Lindgren;Åsa Ljungh;Torkel Wadström
已经从多种哺乳动物和一些禽类的胃、肠道和肝脏中分离出多种螺杆菌，并且已经在人胆汁和肝脏样品中检测到螺杆菌DNA。建立了一种免疫印迹法，以分析自身免疫性肝病患者与健康人相比，对非胃螺杆菌的血清抗体反应。用鸡白痢螺杆菌、胆汁螺杆菌和肝螺杆菌的细胞表面蛋白作为抗原，对36例原发性硬化性胆管炎（PSC）、21例原发性胆汁性肝硬化、19例自身免疫性慢性肝炎和80例献血员的血清进行了免疫印迹分析。在测试之前，血清与幽门螺杆菌的全细胞裂解物交叉吸收。通过光密度扫描测量抗体对这三种螺杆菌的各种蛋白质的反应性，并通过计算机软件处理结果以估计抗原特异性。并与H.幽门。螺杆鸡白痢H. hepaticus对76、30和21 kDa蛋白的反应性以及对H. bilis对22和20 kDa蛋白的反应性似乎具有高特异性。PSC患者血清对H. pullorum、鸡白痢H. bilis和H.在38%、22%和25%的病例中分别发现了肝硬化，在30%、22%和22%的病例中分别发现了其他自身免疫性肝病患者。自身免疫性慢性肝病患者血清非胃螺杆菌抗体阳性率显著高于健康献血员（P < 0.001）。针对肠肝螺杆菌的抗体水平增加，这引起了关于这些新兴细菌病原体在人类自身免疫性肝病中的感染作用的问题。
Various Helicobacter species have been isolated from the stomach, intestinal tract and liver of a variety of mammalian and some avian species, and Helicobacter DNA has been detected in human bile and liver samples. An immunoblot assay was established to analyse serum antibody responses to non-gastric Helicobacter species in patients with autoimmune liver diseases, in comparison with healthy individuals. Sera from 36 patients with primary sclerosing cholangitis (PSC), 21 with primary biliary cirrhosis, 19 with autoimmune chronic hepatitis and 80 blood donors were analysed by immunoblot, using cell-surface proteins from Helicobacter pullorum, Helicobacter bilis and Helicobacter hepaticus as antigens. Prior to testing, sera were cross-absorbed with a whole-cell lysate of Helicobacter pylori. Antibody reactivity to various proteins of these three Helicobacter species was measured by densitometric scanning and results were processed by computer software to estimate antigenic specificity. Results were also compared with antibody response to H. pylori. For H. pullorum, reactivity to at least two of the proteins with molecular masses of 48, 45, 37, 20 and 16 kDa, for H. hepaticus, reactivity to the 76, 30 and 21 kDa proteins and for H. bilis, reactivity to the 22 and 20 kDa proteins, seemed to have high specificity. Positive immunoblot results with sera from patients with PSC to antigens of H. pullorum, H. bilis and H. hepaticus were found in 38, 22 and 25 % of cases, respectively, and from patients with other autoimmune liver diseases, in 30, 22 and 22 % of cases, respectively. Prevalence of serum antibodies to non-gastric Helicobacter species was significantly higher in patients with autoimmune chronic liver diseases than in healthy blood donors (P < 0.001). Increased antibody levels to enterohepatic Helicobacter species raise questions concerning an infectious role of these emerging bacterial pathogens in human autoimmune liver diseases.
影响因子：
29.4
作者：
Fox, JG;Dewhirst, FE;Roa, I
通讯作者：
Roa, I