Indocyanine green fluorescence imaging for evaluation of uterine blood flow in cynomolgus macaque.
Indocyanine green fluorescence imaging for evaluation of uterine blood flow in cynomolgus macaque.
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DOI：
10.1371/journal.pone.0035124
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Aoki D
中科院分区：
文献类型：
--
作者：
Kisu I;Banno K;Mihara M;Lin LY;Tsuji K;Yanokura M;Hara H;Araki J;Iida T;Abe T;Kouyama K;Suganuma N;Aoki D
子宫血流是子宫活力的重要因素，但维持活力所需的血管数量尚不确定。在这项研究中，吲哚菁绿色（ICG）荧光成像检查子宫血流动力学和血管与子宫血流在食蟹猴。从阴道腔切割雌性食蟹猴的子宫，以模拟子宫切除术或子宫移植手术期间的情况，其中仅用子宫和卵巢血管维持子宫灌注。术中采用ICG荧光成像技术观察子宫血流动力学变化。使用成像分析软件绘制时间-强度曲线，以测量所选血管模式的子宫灌注的Tmax。开放手术进行子宫接受营养支持，只有从一侧的子宫血管。术后用经腹超声监测子宫大小。所得到的时间-强度曲线显示了子宫体和子宫颈区域以及整个子宫的平均强度。食蟹猴子宫血流动力学分析显示，子宫血管与子宫灌注显著相关（P = 0.008），而卵巢血管无显著相关性（P = 0.588）。 当子宫血管被夹闭时，卵巢血管达到最大灌注所需的时间延长。术后经腹超声检查显示，术后2个月子宫大小无变化，月经周期恢复。食蟹猴妊娠，胎儿健康良好。子宫血管可能负责子宫血流，甚至一个子宫血管可能足以维持食蟹猴的子宫活力。我们的研究结果表明，ICG荧光成像是有用的子宫血流评价，因为这种方法可以实时观察子宫血流动力学。
Uterine blood flow is an important factor in uterine viability, but the number of blood vessels required to maintain viability is uncertain. In this study, indocyanine green (ICG) fluorescence imaging was used to examine uterine hemodynamics and vessels associated with uterine blood flow in cynomolgus macaque. The uterus of a female cynomolgus macaque was cut from the vaginal canal to mimic a situation during trachelectomy or uterine transplantation surgery in which uterine perfusion is maintained only with uterine and ovarian vessels. Intraoperative uterine hemodynamics was observed using ICG fluorescence imaging under conditions in which various nutrient vessels were selected by clamping of blood vessels. A time-intensity curve was plotted using imaging analysis software to measure the Tmax of uterine perfusion for selected blood vessel patterns. Open surgery was performed with the uterus receiving nutritional support only from uterine vessels on one side. The size of the uterus after surgery was monitored using transabdominal ultrasonography. The resulting time-intensity curves displayed the average intensity in the regions of the uterine corpus and uterine cervix, and in the entire uterus. Analyses of the uterine hemodynamics in the cynomolgus macaque showed that uterine vessels were significantly related to uterine perfusion (P = 0.008), whereas ovarian vessels did not have a significant relationship (P = 0.588). When uterine vessels were clamped, ovarian vessels prolonged the time needed to reach perfusion maximum. Postoperative transabdominal ultrasonography showed that the size of the uterus was not changed 2 months after surgery, with recovery of periodic menstruation. The cynomolgus macaque has got pregnant with favorable fetus well-being. Uterine vessels may be responsible for uterine blood flow, and even one uterine vessel may be sufficient to maintain uterine viability in cynomolgus macaque. Our results show that ICG fluorescence imaging is useful for evaluation of uterine blood flow since this method allows real-time observation of uterine hemodynamics.
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DOI：
10.1196/annals.1335.002
发表时间：
2004-01-01
期刊：
UTERUS AND HUMAN REPRODUCTION
影响因子：
--
作者：
Cicinelli, E;Einer-Jensen, N;Nicoletti, R
通讯作者：
Nicoletti, R
影响因子：
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影响因子：
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DOI：
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发表时间：
2011-01-01
影响因子：
3.8
作者：
Al-Niaimi, Ahmed N.;Einstein, Margaret H.;Kushner, David M.
通讯作者：
Kushner, David M.
影响因子：
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作者：
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