Decreased mRNA levels of cardiac Cx43 and ZO1 in sudden cardiac death related to coronary atherosclerosis: a pilot study
Decreased mRNA levels of cardiac Cx43 and ZO1 in sudden cardiac death related to coronary atherosclerosis: a pilot study
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与冠状动脉粥样硬化相关的心源性猝死中心脏 Cx43 和 ZO1 mRNA 水平降低：一项初步研究
DOI：
10.1007/s00414-016-1353-0
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发表时间：
2016-03
影响因子：
2.1
通讯作者：
Wang Qi
中科院分区：
文献类型：
--
作者：
Xue Ye;Zhao Rui;Du Si-Hao;Zhao Dong;Li Dong-Ri;Xu Jing-Tao;Xie Xiao-Li;Wang Qi
心源性猝死（SCD）是法医实践中最常见的不明原因猝死原因。SCD最常见的原因是与冠状动脉粥样硬化相关的冠状动脉疾病。以往的研究表明，缝隙连接蛋白43（Cx43）和闭锁小带-1（ZO 1）的免疫组化染色可能在心肌缺血的早期诊断中有应用价值。然而，关于其mRNA水平的数据似乎不足。本研究采用法医学尸检材料，包括41例对照组（第1组），32例急性缺血性心脏病相关冠状动脉粥样硬化死亡（第2组），29例创伤性冠状动脉粥样硬化死亡（第3组）的心脏Cx43和ZO 1的mRNA水平。对10例法医尸检左心室组织中的10个候选参考基因进行了检测。EEF 1A 1、PPIA、TPT 1和RPL 13 A被鉴定为最稳定的参考基因。使用这些验证的参考基因，在心脏的双侧心室和心房中检查Cx43和ZO 1的mRNA水平。相对mRNA定量显示组2双侧心室中Cx43和ZO 1的校准标准化相对量（CNRQ）值降低。当使用一个常规参考基因（GAPDH或ACTB）进行标准化时，在三组中几乎没有检测到差异。这些结果表明，心室缝隙连接重构可能是心律失常的一个关键因素。实时荧光定量PCR检测心脏Cx43和ZO 1基因对SCD的诊断有重要意义，而参考基因的验证是关键。
Sudden cardiac death (SCD) is the most frequent cause of sudden unexplained death in forensic practice. The most common cause of SCD is coronary artery disease related to coronary atherosclerosis. Previous study suggested the possible application of connexin 43 (Cx43) and zonula occludens-1 (ZO1) immunostaining in the early diagnosis of myocardial ischemia. However, there appears to be insufficient data with regard to their mRNA levels. The present study investigated the cardiac mRNA levels of Cx43 and ZO1, using forensic autopsy materials consisting of 41 control cases without any disease or structural abnormality of the heart (group 1), 32 deaths due to acute ischemic heart disease related to coronary atherosclerosis without apparent myocardial necrosis (group 2), and 29 traumatic deaths with coronary atherosclerosis (group 3). Ten candidate reference genes were evaluated in the left ventricles of 10 forensic autopsy cases. EEF1A1, PPIA, TPT1, and RPL13A were identified as the most stable reference genes. Using these validated reference genes, mRNA levels of Cx43 and ZO1 were examined in the bilateral ventricles and atria of the heart. Relative mRNA quantification demonstrated decreased calibrated normalized relative quantity (CNRQ) values of Cx43 and ZO1 in bilateral ventricles of group 2. When using one conventional reference gene (GAPDH or ACTB) for normalization, nearly no difference was detected among the three groups. These findings indicate that ventricular gap junction remodeling may be a key contributor to rhythm disturbances. Analysis of cardiac Cx43 and ZO1 using real-time PCR is useful in diagnosis of SCD, and validation of reference genes is crucial.
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影响因子：
1.5
作者：
B. Zhu;T. Ishikawa;T. Michiue;Dongri Li;Dong Zhao;Y. Kamikodai;K. Tsuda;Shuji Okazaki;H. Maeda
通讯作者：
B. Zhu;T. Ishikawa;T. Michiue;Dongri Li;Dong Zhao;Y. Kamikodai;K. Tsuda;Shuji Okazaki;H. Maeda
影响因子：
2.1
作者：
Qi Wang;T. Ishikawa;T. Michiue;B. Zhu;D. Guan;H. Maeda
通讯作者：
Qi Wang;T. Ishikawa;T. Michiue;B. Zhu;D. Guan;H. Maeda
影响因子：
3.7
作者：
de Jonge HJ;Fehrmann RS;de Bont ES;Hofstra RM;Gerbens F;Kamps WA;de Vries EG;van der Zee AG;te Meerman GJ;ter Elst A
通讯作者：
ter Elst A
影响因子：
1.5
作者：
Chappex, Nina;Schlaepfer, Juerg;Michaud, Katarzyna
通讯作者：
Michaud, Katarzyna
影响因子：
4.5
作者：
Carette, Diane;Gilleron, Jerome;Pointis, Georges
通讯作者：
Pointis, Georges