Localization of Swallow‐Green fluorescent protein in Drosophila oogenesis and implications for the role of Swallow in RNA localization
Localization of Swallow‐Green fluorescent protein in Drosophila oogenesis and implications for the role of Swallow in RNA localization
复制标题
燕子绿色荧光蛋白在果蝇卵子发生中的定位及其对燕子在 RNA 定位中的作用的影响
作者：
E. C. Stephenson
报道了一种由燕子和绿色荧光蛋白（GFP）组成的杂交蛋白在果蝇卵发生过程中的定位。我构建了一个带有绿色荧光蛋白的杂交基因插入到燕子的内部位置。该基因被整合到果蝇基因组中，并提供完整的燕子+功能，通过对强燕子突变体的完全拯救进行了检测。Swallow‐GFP定位于卵母细胞皮层的所有点，从卵黄形成阶段到卵母细胞形成结束。在第10期和第11期，卵母细胞的bicoid和htsN4 mRNA运输和卵母细胞定位最为活跃，此时，燕子GFP在卵母细胞前部皮层的浓度高于卵母细胞外侧和卵母细胞后部皮层。在第9期和第12-14期，燕子GFP均匀分布于卵母细胞的前部、外侧和后部皮层。燕子GFP在卵黄形成阶段的位置与使用抗燕子抗体的间接免疫荧光测定的内源性燕子蛋白的位置相同。在卵母细胞皮层，swallow - GFP存在于密集的皮质肌动蛋白网络内部或内部的颗粒结构中。这些颗粒很少或没有运动，表明它们附着或嵌入卵母细胞皮层。这些观察结果最容易在mRNA锚定或燕子蛋白的微管组织功能的背景下解释。创世纪39:280-287,2004。©2004 Wiley‐Liss, Inc。
The localization of a hybrid protein composed of swallow and Green Fluorescent Protein (GFP) during Drosophila oogenesis is reported. I constructed a hybrid gene with GFP inserted into an internal position of swallow. This gene was integrated into the Drosophila genome and provides full swallow+ function, as assayed by the complete rescue of strong swallow mutants. Swallow‐GFP is localized at all points along the oocyte cortex from vitellogenic stages of oogenesis through the end of oogenesis. Higher concentrations of swallow‐GFP are present at the anterior oocyte cortex than at the lateral and posterior oocyte cortices at Stages 10 and 11, when bicoid and htsN4 mRNA transport from nurse cells and localization in the oocyte are most active. At Stage 9 and at Stages 12–14 swallow‐GFP is equally distributed at the anterior, lateral, and posterior oocyte cortices. The position of swallow‐GFP in vitellogenic stages is identical to the position of endogenous swallow protein determined by indirect immunofluorescence using an anti‐swallow antibody. At the oocyte cortex, swallow‐GFP is present in particulate structures that lie within or just internal to the dense cortical actin meshwork. These particles show little or no movement, suggesting that they are attached to or embedded in the oocyte cortex. These observations are most easily interpreted in the context of mRNA anchoring or microtubule organizing functions for the swallow protein. genesis 39:280–287, 2004. © 2004 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.90.6.2512
发表时间：
1993-03-15
影响因子：
11.1
作者：
DING, D;PARKHURST, SM;LIPSHITZ, HD
通讯作者：
LIPSHITZ, HD
果蝇卵室和胚胎中吞咽蛋白的分布。
DOI：
10.1242/dev.119.2.457
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hegdé,J;Stephenson,EC
通讯作者：
Stephenson,EC
果蝇卵子发生过程中细胞质运输和细胞骨架组织的体内分析：多步前定位途径的表征。
DOI：
10.1242/dev.125.18.3655
发表时间：
1998
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Theurkauf,WE;Hazelrigg,TI
通讯作者：
Hazelrigg,TI