Evolution and Expression of Paxillin Genes in Teleost Fish.
Evolution and Expression of Paxillin Genes in Teleost Fish.
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DOI：
10.1371/journal.pone.0165266
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Amack JD
中科院分区：
文献类型：
--
作者：
Jacob AE;Turner CE;Amack JD
桩蛋白家族蛋白调节细胞外基质粘附下游的细胞内信号传导。桩蛋白在胚胎发育过程中的组织表达模式和细胞功能仍然知之甚少。此外，该基因家族的进化尚未得到彻底研究。本报告描述了一种新的桩蛋白基因，称为桩蛋白b，在硬骨鱼的进化和表达。比对表明硬骨鱼Paxillin-a和Paxillin-b蛋白彼此高度同源并且与人Paxillin高度同源。系统发育和同线性分析表明，这些基因起源于一个祖先桩蛋白基因，是在一个共同的祖先硬骨鱼和四足动物的重复。使用斑马鱼对Paxillin-a和Paxillin-b的时空表达谱的分析揭示了在胚胎发育期间Paxillin-a和Paxillin-b的重叠和不同的域。在哺乳动物细胞中表达的斑马鱼桩蛋白直向同源物的定位表明，这两种蛋白质定位于粘着斑，类似于哺乳动物桩蛋白。这表明这些蛋白质调节其内源性组织中的粘附依赖性过程。Paxillin-a和Paxillin-b在硬骨鱼中通过复制产生。这些基因可能在其他生物体中发挥与桩蛋白基因类似的作用。这项工作提供了一个框架，在开发过程中使用斑马鱼作为体内模型系统的桩蛋白家族成员的功能研究。
Paxillin family proteins regulate intracellular signaling downstream of extracellular matrix adhesion. Tissue expression patterns and cellular functions of Paxillin proteins during embryo development remain poorly understood. Additionally, the evolution of this gene family has not been thoroughly investigated. This report characterizes the evolution and expression of a novel Paxillin gene, called Paxillin-b, in Teleosts. Alignments indicate that Teleost Paxillin-a and Paxillin-b proteins are highly homologous to each other and to human Paxillin. Phylogenetic and synteny analyses suggest that these genes originated from the duplication of an ancestral Paxillin gene that was in a common ancestor of Teleosts and Tetrapods. Analysis of the spatiotemporal expression profiles of Paxillin-a and Paxillin-b using zebrafish revealed both overlapping and distinct domains for Paxillin-a and Paxillin-b during embryo development. Localization of zebrafish Paxillin orthologs expressed in mammalian cells demonstrated that both proteins localize to focal adhesions, similar to mammalian Paxillin. This suggests these proteins regulate adhesion-dependent processes in their endogenous tissues. Paxillin-a and Paxillin-b were generated by duplication in Teleosts. These genes likely play similar roles as Paxillin genes in other organisms. This work provides a framework for functional investigation of Paxillin family members during development using the zebrafish as an in vivo model system.
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