Optogenetic activation of septal GABAergic afferents entrains neuronal firing in the medial habenula.
Optogenetic activation of septal GABAergic afferents entrains neuronal firing in the medial habenula.
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DOI：
10.1038/srep34800
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发表时间：
2016-10-05
影响因子：
4.6
通讯作者：
Shin KS
中科院分区：
文献类型：
--
作者：
Choi K;Lee Y;Lee C;Hong S;Lee S;Kang SJ;Shin KS
内侧缰核（MHb）在尼古丁相关行为如尼古丁厌恶和戒断中起重要作用。MHb接受来自Broca内侧隔/斜角带（MS/DB）的GABA能输入，但调节MHb活性的突触机制尚不清楚。GABA（γ -氨基丁酸）是激活GABAA受体和GABAB受体的主要抑制性神经递质。然而，依赖于细胞内氯离子浓度，GABAA受体也以兴奋性方式起作用。在缺乏各种突触输入的情况下，我们发现MHb神经元以约4.4 Hz的速率显示自发强直放电。光遗传刺激MS/DB输入MHb诱发GABAA受体介导的突触电流，产生刺激锁定的神经元放电。随后GABAB受体的延迟但持久的激活减弱了内在紧张性放电。因此，单独的隔GABA能输入协调兴奋性GABAA和抑制性GABAB受体，从而引起MHb神经元的放电。
The medial habenula (MHb) plays an important role in nicotine-related behaviors such as nicotine aversion and withdrawal. The MHb receives GABAergic input from the medial septum/diagonal band of Broca (MS/DB), yet the synaptic mechanism that regulates MHb activity is unclear. GABA (γ -aminobutyric acid) is a major inhibitory neurotransmitter activating both GABAA receptors and GABAB receptors. Depending on intracellular chloride concentration, however, GABAA receptors also function in an excitatory manner. In the absence of various synaptic inputs, we found that MHb neurons displayed spontaneous tonic firing at a rate of about ~4.4 Hz. Optogenetic stimulation of MS/DB inputs to the MHb evoked GABAA receptor-mediated synaptic currents, which produced stimulus-locked neuronal firing. Subsequent delayed yet lasting activation of GABAB receptors attenuated the intrinsic tonic firing. Consequently, septal GABAergic input alone orchestrates both excitatory GABAA and inhibitory GABAB receptors, thereby entraining the firing of MHb neurons.
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