Laminarin counteracts diet-induced obesity associated with glucagon-like peptide-1 secretion.
Laminarin counteracts diet-induced obesity associated with glucagon-like peptide-1 secretion.
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海带多糖可抵消与胰高血糖素样肽-1 分泌相关的饮食引起的肥胖
DOI：
10.18632/oncotarget.19957
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发表时间：
2017-11-21
期刊：
影响因子：
--
通讯作者：
Shu G
中科院分区：
文献类型：
--
作者：
Yang L;Wang L;Zhu C;Wu J;Yuan Y;Yu L;Xu Y;Xu J;Wang T;Liao Z;Wang S;Zhu X;Gao P;Zhang Y;Wang X;Jiang Q;Shu G
层粘连蛋白是一种从褐海藻中分离出来的β-葡聚糖，具有一定的生理活性，包括免疫调节和抗肿瘤功能。为了研究层粘连蛋白对能量稳态的影响，我们给小鼠口服层粘连蛋白来测试食物摄入、脂肪沉积和葡萄糖稳态。长期使用laminarin治疗可显著降低高脂肪饮食引起的体重增加和脂肪沉积，降低血糖水平和葡萄糖耐量。层粘连蛋白显著提高血清胰高血糖素样肽-1 （GLP-1）含量及回肠胰高血糖素原和激素转化酶原1 mRNA表达水平。随后，laminarin抑制小鼠的食物摄取量、下丘脑AgRP神经元活性和AgRP表达，但激活胰腺功能。此外，laminarin诱导的食欲下降被Exendin（9-39）完全阻断，Exendin是GLP-1受体的特异性竞争性抑制剂。然后采用STC-1细胞研究层粘胶蛋白促进GLP-1体外分泌的潜在机制。结果显示，层粘菌素剂量依赖性地促进STC-1细胞GLP-1分泌和c-Fos蛋白表达，且不依赖于Dectin-1和CD18。有趣的是，BAPTA-AM，一种钙螯合剂，能有效地减弱层压蛋白诱导的[Ca2+]i升高、c-Fos表达和GLP-1分泌。总之，我们的数据支持层粘菌素抵消饮食引起的肥胖，并通过[Ca2+]i刺激GLP-1分泌；这一发现为层粘菌素用于治疗肥胖和维持葡萄糖稳态提供了实验依据。
Laminarin, a type of β-glucan isolated from brown seaweeds, exhibits verity of physiological activities, which include immunology modulation and antitumor function. To investigate the effect of laminarin on energy homeostasis, mice were orally administrated with laminarin to test food intake, fat deposition, and glucose homeostasis. Chronically, laminarin treatment significantly decreases high-fat-diet-induced body weight gain and fat deposition and reduces blood glucose level and glucose tolerance. Acutely, laminarin enhances serum glucagon-like peptide-1 (GLP-1) content and the mRNA expression level of proglucagon and prohormone convertase 1 in ileum. Subsequently, laminarin suppresses the food intake of mice, the hypothalamic AgRP neuron activity, and AgRP expression but activates pancreatic function. Furthermore, laminarin-induced appetite reduction was totally blocked by Exendin (9-39), a specific competitive inhibitor of GLP-1 receptor. Then, STC-1 cells were adopted to address the underlying mechanism, by which laminarin promoted GLP-1 secretion in vitro. Results showed that laminarin dose-dependently promoted GLP-1 secretion and c-Fos protein expression in STC-1 cells, which were independent of Dectin-1 and CD18. Interestingly, BAPTA-AM, a calcium-chelating agent, potently attenuated laminarin-induced [Ca2+]i elevation, c-Fos expression, and GLP-1 secretion. In summary, our data support that laminarin counteracts diet-induced obesity and stimulates GLP-1 secretion via [Ca2+]i; this finding provides an experimental basis for laminarin application to treat obesity and maintain glucose homeostasis.
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影响因子：
--
作者：
Dharmalingam M;Sriram U;Baruah MP
通讯作者：
Baruah MP
影响因子：
7.7
作者：
Arakawa M;Mita T;Azuma K;Ebato C;Goto H;Nomiyama T;Fujitani Y;Hirose T;Kawamori R;Watada H
通讯作者：
Watada H
影响因子：
7.7
作者：
Gribble, FM;Williams, L;Reimann, F
通讯作者：
Reimann, F
影响因子：
56.9
作者：
Chawla, S;Hardingham, GE;Bading, H
通讯作者：
Bading, H
影响因子：
2.2
作者：
Fuentes, Ana-Lucia;Millis, Leonard;Sigola, Lynette
通讯作者：
Sigola, Lynette