The absence or presence of a lytic coliphage affects the response of Escherichia coli to heat, chlorine, or UV exposure
The absence or presence of a lytic coliphage affects the response of Escherichia coli to heat, chlorine, or UV exposure
复制标题
裂解性大肠杆菌噬菌体的存在或不存在会影响大肠杆菌对热、氯或紫外线暴露的反应
DOI：
10.1007/s12223-018-0600-9
复制
发表时间：
2018
影响因子：
2.6
通讯作者：
Ameh E
中科院分区：
文献类型：
--
作者：
Ameh E
消毒的目的是最大限度地灭活目标生物并持续抑制其再生。尽管许多消毒工作在处理后直接测量效果时实现了有效的灭活，但这种效果的可持续性存在问题。一方面是经过处理的细菌可能会恢复并重新获得生长能力。在环境背景下，另一个问题是存活的细菌如何适应无所不在的噬菌体的捕食。临时数据表明，细菌在受到亚致死热应激时可能会产生噬菌体抗性表型。这一结果让我们想知道暴露于氯和UV-LED消毒强度梯度的细菌对噬菌体介导的裂解的敏感性。而暴露于低亚致死氯剂量的细菌仍然经历噬菌体介导的裂解，临界氯Ct为0.5毫克分钟/升消除这种敏感性和诱导噬菌体抗性的细胞存活治疗。在UV的情况下，即使是最小的测试剂量2.8 mJ/cm 2也能消除噬菌体裂解，导致直接再生长。结果表明，与未处理的细胞相比，在消毒后存活的细菌在处理后直接具有更高的环境存活机会。一个原因可能在于它们的代谢受损，这对噬菌体复制至关重要。
Disinfection aims at maximal inactivation of target organisms and the sustainable suppression of their regrowth. Whereas many disinfection efforts achieve efficient inactivation when the effect is measured directly after treatment, there are questions about the sustainability of this effect. One aspect is that the treated bacteria might recover and regain the ability to grow. In an environmental context, another question is how amenable surviving bacteria are to predation by omnipresent bacteriophages. Provisional data suggested that bacteria when subjected to sublethal heat stress might develop a phage-resistant phenotype. The result made us wonder about the susceptibility to phage-mediated lysis for bacteria exposed to a gradient of chlorine and UV-LED disinfection strengths. Whereas bacteria exposed to low sublethal chlorine doses still underwent phage-mediated lysis, the critical chlorine Ct of 0.5 mg min/L eliminated this susceptibility and induced phage resistance in the cells that survived treatment. In the case of UV, even the smallest tested dose of 2.8 mJ/cm2abolished phage lysis leading to direct regrowth. Results suggest that bacteria surviving disinfection might have higher environmental survival chances directly after treatment compared to non-treated cells. A reason could possibly lie in their compromised metabolism that is essential for phage replication.
登录
查看更多内容
影响因子：
2.9
作者：
Awais, Raheela;Fukudomi, Hiroshi;Tanji, Yasunori
通讯作者：
Tanji, Yasunori
紫外线消毒后水中大肠杆菌回收的重要性
影响因子：
2.2
作者：
Z. Bohrerova;J. Rosenblum;K. Linden
通讯作者：
K. Linden
在没有光和暗修复的情况下，紫外线消毒后细菌的再生潜力。
影响因子：
2.2
作者：
Kerim Kollu;B. Örmeci
通讯作者：
B. Örmeci
颗粒聚集微生物对紫外线消毒的影响。
影响因子：
2.2
作者：
H. Mamane;K. Linden
通讯作者：
K. Linden
氯灭活微生物的动力学——II 需氯时的动力学
影响因子：
12.8
作者：
C. Haas;S. B. Karra
通讯作者：
S. B. Karra