Transcriptome sequencing of the blind subterranean mole rat, Spalax galili: utility and potential for the discovery of novel evolutionary patterns.
Transcriptome sequencing of the blind subterranean mole rat, Spalax galili: utility and potential for the discovery of novel evolutionary patterns.
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DOI：
10.1371/journal.pone.0021227
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Band M
中科院分区：
文献类型：
--
作者：
Malik A;Korol A;Hübner S;Hernandez AG;Thimmapuram J;Ali S;Glaser F;Paz A;Avivi A;Band M
盲的地下鼹鼠（Spalax ehrenbergi superspecies）是极端环境下生存的模式动物，因为它能够在严重的缺氧压力和黑暗下生活在地下栖息地。在这里，我们报告的转录组测序的Spalax galili，染色体类型的S。埃伦贝格。使用桑格、GS FLX和GS FLX钛技术对从暴露于低氧和常氧条件的动物中分离的肌肉和脑组织的cDNA库进行测序。序列的组装产生了超过51，000个与约12，000个小鼠、大鼠或人类基因同源的isotig。基于这些结果，有可能检测到大量的剪接变体、SNP和新的转录区域。此外，在组织和处理之间检测到多种差异表达模式。这里提出的结果将作为一个有价值的资源，为未来的研究，旨在确定基因和基因区域的适应性辐射与盲鼹鼠的地下生活过程中演变。
The blind subterranean mole rat (Spalax ehrenbergi superspecies) is a model animal for survival under extreme environments due to its ability to live in underground habitats under severe hypoxic stress and darkness. Here we report the transcriptome sequencing of Spalax galili, a chromosomal type of S. ehrenbergi. cDNA pools from muscle and brain tissues isolated from animals exposed to hypoxic and normoxic conditions were sequenced using Sanger, GS FLX, and GS FLX Titanium technologies. Assembly of the sequences yielded over 51,000 isotigs with homology to ∼12,000 mouse, rat or human genes. Based on these results, it was possible to detect large numbers of splice variants, SNPs, and novel transcribed regions. In addition, multiple differential expression patterns were detected between tissues and treatments. The results presented here will serve as a valuable resource for future studies aimed at identifying genes and gene regions evolved during the adaptive radiation associated with underground life of the blind mole rat.
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影响因子：
8
作者：
Avivi, A.;Ashur-Fabian, O.;Nevo, E.
通讯作者：
Nevo, E.
影响因子：
30.8
作者：
Boyadjiev, Simeon A.;Fromme, J. Christopher;Eyaid, Wafaa
通讯作者：
Eyaid, Wafaa
DOI：
10.1086/physzool.54.1.30155800
发表时间：
1981-01-01
期刊：
PHYSIOLOGICAL ZOOLOGY
影响因子：
--
作者：
ARIELI, R;AR, A
通讯作者：
AR, A
DOI：
10.1073/pnas.0404998101
发表时间：
2004-08-17
影响因子：
11.1
作者：
Ashur-Fabian, C;Avivi, A;Rechavi, G
通讯作者：
Rechavi, G
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y