Investigating the Fibrillar Ultrastructure and Mechanics in Keloid Scars Using In Situ Synchrotron X-ray Nanomechanical Imaging.
Investigating the Fibrillar Ultrastructure and Mechanics in Keloid Scars Using In Situ Synchrotron X-ray Nanomechanical Imaging.
复制标题
DOI：
10.3390/ma15051836
复制
发表时间：
2022-03-01
期刊：
影响因子：
--
通讯作者：
Gupta HS
中科院分区：
文献类型：
--
作者：
Zhang Y;Hollis D;Ross R;Snow T;Terrill NJ;Lu Y;Wang W;Connelly J;Tozzi G;Gupta HS
纤维化瘢痕形成在一系列胶原组织疾病中普遍存在。了解基质生物物理学在纤维化进展中的作用对于开发治疗方法以及阐明生物学机制非常重要。在这里，我们演示了如何微焦点小角度X射线散射（SAXS），在原位力学和相关成像，可以提供定量和位置分辨的纤维化基质纳米结构及其机械性能的信息。我们以皮肤瘢痕疙瘩为例。SAXS映射揭示了胶原纤维浓度、纤维预应变（D期变化）和可能与蛋白聚糖相关的新纤维间组分的异质梯度，表明纳米级复杂3D结构的证据。此外，我们证明了一个衍射对比相关成像技术的原理证明，纳入，第一次，DIC和SAXS，并提供了一个初步的估计，用于测量空间分辨原纤维水平的应变和重定向在这样的异质组织。通过应用该方法，我们量化了（在微观尺度上）原纤维的重新取向、原纤维D周期方差的增加以及在~ 20%的宏观组织应变下平均D周期的增加。我们的研究结果打开了机会，使用同步辐射X射线纳米力学成像作为一种定量工具，探测瘢痕疙瘩和其他纤维化疾病的结构-功能关系原位。
Fibrotic scarring is prevalent in a range of collagenous tissue disorders. Understanding the role of matrix biophysics in contributing to fibrotic progression is important to develop therapies, as well as to elucidate biological mechanisms. Here, we demonstrate how microfocus small-angle X-ray scattering (SAXS), with in situ mechanics and correlative imaging, can provide quantitative and position-resolved information on the fibrotic matrix nanostructure and its mechanical properties. We use as an example the case of keloid scarring in skin. SAXS mapping reveals heterogeneous gradients in collagen fibrillar concentration, fibril pre-strain (variations in D-period) and a new interfibrillar component likely linked to proteoglycans, indicating evidence of a complex 3D structure at the nanoscale. Furthermore, we demonstrate a proof-of-principle for a diffraction-contrast correlative imaging technique, incorporating, for the first time, DIC and SAXS, and providing an initial estimate for measuring spatially resolved fibrillar-level strain and reorientation in such heterogeneous tissues. By application of the method, we quantify (at the microscale) fibrillar reorientations, increases in fibrillar D-period variance, and increases in mean D-period under macroscopic tissue strains of ~20%. Our results open the opportunity of using synchrotron X-ray nanomechanical imaging as a quantitative tool to probe structure–function relations in keloid and other fibrotic disorders in situ.
登录
查看更多内容
DOI：
10.1016/j.jmbbm.2015.03.010
发表时间：
2015-12-01
影响因子：
3.9
作者：
Bertinetti, Luca;Masic, Admir;Fratzl, Peter
通讯作者：
Fratzl, Peter
影响因子：
--
作者：
Barallobre-Barreiro J;Woods E;Bell RE;Easton JA;Hobbs C;Eager M;Baig F;Ross AM;Mallipeddi R;Powell B;Soldin M;Mayr M;Shaw TJ
通讯作者：
Shaw TJ
影响因子：
3.2
作者：
Dall'Ara, Enrico;Pena-Fernandez, Marta;Tozzi, Gianluca
通讯作者：
Tozzi, Gianluca
影响因子：
5.7
作者：
Gauglitz, Gerd G.;Korting, Hans C.;Jeschke, Marc G.
通讯作者：
Jeschke, Marc G.
影响因子：
3
作者：
Almer, JD;Stock, SR
通讯作者：
Stock, SR