Self-incompatibility requires GPI anchor remodeling by the poppy PGAP1 ortholog HLD1.
Self-incompatibility requires GPI anchor remodeling by the poppy PGAP1 ortholog HLD1.
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DOI：
10.1016/j.cub.2022.02.072
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发表时间：
2022-05-09
期刊：
影响因子：
--
通讯作者：
Nowack MK
中科院分区：
文献类型：
--
作者：
Lin Z;Xie F;Triviño M;Zhao T;Coppens F;Sterck L;Bosch M;Franklin-Tong VE;Nowack MK
糖基磷脂酰肌醇锚定蛋白（GPI-AP）被拴系到质膜的外小叶，在那里它们作为真核生物中大量生物过程的关键调节剂发挥作用。自交不亲和性（SI）通过识别和排斥“自花”花粉在高等植物的受精过程中起着关键作用。在这里，我们使用拟南芥线工程是自交不亲和的表达虞美人SI决定簇的SI抑制筛选。我们将HLD 1/AtPGAP 1（人GPI-肌醇脱酰酶PGAP 1的直系同源物）确定为SI反应所需的关键成分。除了延迟开花时间，没有发育缺陷，观察hld 1/atpgap 1基因敲除植物，但SI被完全废除。我们证明，HLD 1/AtPGAP 1作为一个GPI-肌醇脱酰酶的功能，这种GPI-重塑活动是必不可少的SI。使用GFP-SKU 5作为代表性GPI-AP，我们表明HLD 1/AtPGAP 1突变不影响GPI-AP的产生和靶向，但影响其在体内的切割和从膜释放。我们的数据不仅牵连GPI-AP在SI，提供了新的方向，调查SI机制，但也确定了一个关键的功能作用GPI-AP重塑肌醇脱酰在植物。
Glycosylphosphatidylinositol anchored proteins (GPI-APs) are tethered to the outer leaflet of the plasma membrane where they function as key regulators of a plethora of biological processes in eukaryotes. Self-incompatibility (SI) plays a pivotal role regulating fertilization in higher plants through recognition and rejection of ‘self’ pollen. Here we used Arabidopsis thaliana lines engineered to be self-incompatible by expression of Papaver rhoeas SI determinants for an SI suppressor screen. We identify HLD1/AtPGAP1, an ortholog of the human GPI-inositol deacylase PGAP1, as a critical component required for the SI response. Besides a delay in flowering time, no developmental defects were observed in hld1/atpgap1 knockout plants, but SI was completely abolished. We demonstrate that HLD1/AtPGAP1 functions as a GPI-inositol deacylase and that this GPI-remodeling activity is essential for SI. Using GFP–SKU5 as a representative GPI-AP, we show that the HLD1/AtPGAP1 mutation does not affect GPI-AP production and targeting but affects their cleavage and release from membranes in vivo. Our data not only implicate GPI-APs in SI, providing new directions to investigate SI mechanisms, but also identify a key functional role for GPI-AP remodeling by inositol deacylation in planta.
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影响因子：
2.9
作者：
Abas L;Luschnig C
通讯作者：
Luschnig C
影响因子：
14.9
作者：
Ding, Y;Chan, CY;Lawrence, CE
通讯作者：
Lawrence, CE
拟南芥花粉管完整性和精子释放受 RALF 介导的信号调节
DOI：
10.1126/science.aao3642
发表时间：
2017-12-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ge Z;Bergonci T;Zhao Y;Zou Y;Du S;Liu MC;Luo X;Ruan H;García-Valencia LE;Zhong S;Hou S;Huang Q;Lai L;Moura DS;Gu H;Dong J;Wu HM;Dresselhaus T;Xiao J;Cheung AY;Qu LJ
通讯作者：
Qu LJ
影响因子：
11.6
作者：
Gillmor, CS;Lukowitz, W;Somerville, C
通讯作者：
Somerville, C
影响因子：
7.4
作者：
Bundy, Mark G. R.;Kosentka, Pawel Z.;Shpak, Elena D.
通讯作者：
Shpak, Elena D.