Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling.

Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling.
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拟南芥花粉管完整性和精子释放受 RALF 介导的信号调节

DOI:
10.1126/science.aao3642
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发表时间:
2017-12-22
期刊:
Science (New York, N.Y.)
影响因子:
--
通讯作者:
Qu LJ
Qu LJ
中科院分区:
其他
文献类型:
--
作者:
Ge Z;Bergonci T;Zhao Y;Zou Y;Du S;Liu MC;Luo X;Ruan H;García-Valencia LE;Zhong S;Hou S;Huang Q;Lai L;Moura DS;Gu H;Dong J;Wu HM;Dresselhaus T;Xiao J;Cheung AY;Qu LJ

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在开花植物中,受精需要在花粉管和雌性生殖组织之间进行复杂的细胞间通讯,这是由细胞外信号分子与花粉管表面受体相互作用控制的。我们发现拟南芥中的两个这样的受体BUPS 1和BUPS 2以及它们的肽配体RALF 4和RALF 19是花粉管表达的,并且是维持花粉管完整性所必需的。BUPS 1和BUPS 2通过其胞外域与受体ANXUR 1和ANXUR 2相互作用,并且两组受体都结合RALF 4和RALF 19。这些受体-配体相互作用与雌性衍生的配体RALF 34竞争,其在纳摩尔浓度下诱导花粉管破裂。我们认为,RALF 34取代RALF 4和RALF 19在接口的花粉管雌配子体接触,从而解除BUPS-ANXUR信号,反过来导致花粉管破裂和精子释放。
In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface. We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube–expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations. We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube–female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.
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