Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system.
Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system.
复制标题
DOI：
10.1186/1297-9716-44-40
复制
发表时间：
2013-06-11
影响因子：
4.4
通讯作者：
Rainard P
中科院分区：
文献类型：
--
作者：
Gilbert FB;Cunha P;Jensen K;Glass EJ;Foucras G;Robert-Granié C;Rupp R;Rainard P
由大肠杆菌和金黄色葡萄球菌引起的乳腺炎是奶牛的主要病理学。为了更好地理解乳腺对这两种病原体的不同反应，我们用大肠杆菌或金黄色葡萄球菌刺激牛乳腺上皮细胞（bMEC）。coli粗脂多糖（LPS）或S.金黄色葡萄球菌培养上清液（SaS），以比较初始bMEC应答的转录组谱。通过使用HEK 293报告细胞作为模式识别受体，发现LPS制剂刺激TLR 2和TLR 4，但不刺激TLR 5、Nod 1或Nod 2，而SaS刺激TLR 2。生化分析表明，脂磷壁酸、蛋白A和α-溶血素均存在于SaS中，并且发现bMEC对这些分子中的每一个都有反应。转录组分析显示LPS和SaS部分共享核心先天免疫应答。然而，LPS诱导的基因表达显著高于SaS诱导的基因表达，并且倍数变化的幅度更大。微阵列数据分析表明，活化途径和早期趋化因子和细胞因子的产生先于防御和应激反应。主要的不同应答是LPS而不是SaS对I型IFN途径的激活。LPS对单核白细胞趋化因子（Cxcl 10、Ccl 2、Ccl 5和Ccl 20）的上调高于SaS，这可能与I型IFN途径的不同激活有关，并可诱导白细胞初始募集的不同特征。MEC对两种刺激的反应不同，LPS与NF-κB和Fas信号通路相关，而SaS与AP-1和IL-17 A信号通路相关。值得注意的是，在蛋白水平上，TNF-α和IL-1β的分泌均未被任一刺激物诱导。这些结果提示，MEC对E. coli和革兰氏阳性菌S.金黄色葡萄球菌引起了具有不同白细胞募集和乳腺对感染的不同炎性和先天免疫反应的应答的开始。
Mastitis caused by Escherichia coli and Staphylococcus aureus is a major pathology of dairy cows. To better understand the differential response of the mammary gland to these two pathogens, we stimulated bovine mammary epithelial cells (bMEC) with either E. coli crude lipopolysaccharide (LPS) or with S. aureus culture supernatant (SaS) to compare the transcriptomic profiles of the initial bMEC response. By using HEK 293 reporter cells for pattern recognition receptors, the LPS preparation was found to stimulate TLR2 and TLR4 but not TLR5, Nod1 or Nod2, whereas SaS stimulated TLR2. Biochemical analysis revealed that lipoteichoic acid, protein A and α-hemolysin were all present in SaS, and bMEC were found to be responsive to each of these molecules. Transcriptome profiling revealed a core innate immune response partly shared by LPS and SaS. However, LPS induced expression of a significant higher number of genes and the fold changes were of greater magnitude than those induced by SaS. Microarray data analysis suggests that the activation pathways and the early chemokine and cytokine production preceded the defense and stress responses. A major differential response was the activation of the type I IFN pathway by LPS but not by SaS. The higher upregulation of chemokines (Cxcl10, Ccl2, Ccl5 and Ccl20) that target mononuclear leucocytes by LPS than by SaS is likely to be related to the differential activation of the type I IFN pathway, and could induce a different profile of the initial recruitment of leucocytes. The MEC responses to the two stimuli were different, as LPS was associated with NF-κB and Fas signaling pathways, whereas SaS was associated with AP-1 and IL-17A signaling pathways. It is noteworthy that at the protein level secretion of TNF-α and IL-1β was not induced by either stimulus. These results suggest that the response of MEC to diffusible stimuli from E. coli and S. aureus contributes to the onset of the response with differential leucocyte recruitment and distinct inflammatory and innate immune reactions of the mammary gland to infection.
登录
查看更多内容
DOI：
10.1186/1297-9686-43-24
发表时间：
2011-06-24
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Brand B;Hartmann A;Repsilber D;Griesbeck-Zilch B;Wellnitz O;Kühn C;Ponsuksili S;Meyer HH;Schwerin M
通讯作者：
Schwerin M
DOI：
10.1152/ajplung.90498.2008
发表时间：
2009-03-01
影响因子：
4.9
作者：
Below, Sabine;Konkel, Anne;Hildebrandt, Jan-Peter
通讯作者：
Hildebrandt, Jan-Peter
影响因子：
3.8
作者：
Bougarn, Salim;Cunha, Patricia;Rainard, Pascal
通讯作者：
Rainard, Pascal
DOI：
10.2165/00066982-200307030-00008
发表时间：
2003-01-01
期刊：
Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology
影响因子：
--
作者：
Bottero, Virginie;Imbert, Veronique;Peyron, Jean-Francois
通讯作者：
Peyron, Jean-Francois
影响因子：
3.3
作者：
Bannerman, D. D.
通讯作者：
Bannerman, D. D.