Impact of dietary gluten on regulatory T cells and Th17 cells in BALB/c mice.
Impact of dietary gluten on regulatory T cells and Th17 cells in BALB/c mice.
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DOI：
10.1371/journal.pone.0033315
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Funda DP
中科院分区：
文献类型：
--
作者：
Antvorskov JC;Fundova P;Buschard K;Funda DP
饮食麸质影响1型糖尿病（T1 D）的发展，无麸质（GF）饮食对T1 D的发展具有保护作用。由于麸质对肠道免疫的直接影响，它可能会影响T1 D;然而，这些机制尚未得到充分研究。我们研究了GF饮食与含麸质的标准（STD）饮食相比对BALB/c小鼠中选定的T细胞亚群的影响，这些T细胞亚群与调节功能以及促炎性Th 17细胞相关。此外，我们评估了饮食诱导的各种T细胞标志物表达的变化，并确定变化是否仅限于肠道或非肠道淋巴室。含麸质的STD饮食导致研究的所有淋巴区室中γδ T细胞的比例显著降低，尽管在一些γδ T细胞亚群（CD 8+，CD 103+）中检测到增加。此外，它还降低了派尔集合淋巴结中CD 4 + CD 62 L + T细胞的比例。有趣的是，在CD 4 + Foxp 3 + T细胞或CD 3 + CD 49 b+细胞（NKT细胞）和CD 3 − CD 49 b+（NK）细胞中没有发现饮食诱导的变化。喂食STD饮食的小鼠在粘膜和非粘膜区室中显示出CD 4 + CD 45 RB高+和CD 103 + T细胞的比例增加以及CD 4 + CD 45 RB低+ T细胞的比例降低。与自身免疫发展相关的Th 17细胞群在喂食STD饮食的小鼠的胰腺淋巴结中显著增加。总的来说，我们的数据表明，饮食麸质影响多种调节性T细胞亚群以及粘膜淋巴组织中的Th 17细胞，而在非粘膜淋巴区室中观察到的差异较小。
Dietary gluten influences the development of type 1 diabetes (T1D) and a gluten-free (GF) diet has a protective effect on the development of T1D. Gluten may influence T1D due to its direct effect on intestinal immunity; however, these mechanisms have not been adequately studied. We studied the effect of a GF diet compared to a gluten-containing standard (STD) diet on selected T cell subsets, associated with regulatory functions as well as proinflammatory Th17 cells, in BALB/c mice. Furthermore, we assessed diet-induced changes in the expression of various T cell markers, and determined if changes were confined to intestinal or non-intestinal lymphoid compartments. The gluten-containing STD diet led to a significantly decreased proportion of γδ T cells in all lymphoid compartments studied, although an increase was detected in some γδ T cell subsets (CD8+, CD103+). Further, it decreased the proportion of CD4+CD62L+ T cells in Peyer's patches. Interestingly, no diet-induced changes were found among CD4+Foxp3+ T cells or CD3+CD49b+cells (NKT cells) and CD3−CD49b+ (NK) cells. Mice fed the STD diet showed increased proportions of CD4+CD45RBhigh+ and CD103+ T cells and a lower proportion of CD4+CD45RBlow+ T cells in both mucosal and non-mucosal compartments. The Th17 cell population, associated with the development of autoimmunity, was substantially increased in pancreatic lymph nodes of mice fed the STD diet. Collectively, our data indicate that dietary gluten influences multiple regulatory T cell subsets as well as Th17 cells in mucosal lymphoid tissue while fewer differences were observed in non-mucosal lymphoid compartments.
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影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
气溶胶胰岛素诱导调节性CD8伽马三角细胞，可预防鼠胰岛素依赖性糖尿病。
DOI：
10.1084/jem.184.6.2167
发表时间：
1996-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Harrison LC;Dempsey-Collier M;Kramer DR;Takahashi K
通讯作者：
Takahashi K
影响因子：
2.5
作者：
Gorfu G;Rivera-Nieves J;Ley K
通讯作者：
Ley K
影响因子：
8
作者：
Funda, David P.;Kaas, Anne;Buschard, Karsten
通讯作者：
Buschard, Karsten
影响因子：
3.8
作者：
Chabaud, M;Garnero, P;Miossec, P
通讯作者：
Miossec, P