Enhancer activity of HS2 of the human beta-LCR is modulated by distance from the key nucleosome.
Enhancer activity of HS2 of the human beta-LCR is modulated by distance from the key nucleosome.
复制标题
人类 β-LCR 的 HS2 增强子活性受距关键核小体的距离调节。
作者：
Y. Onishi;R. Kiyama
一类弯曲DNA普遍存在于真核基因组DNA中，平均距离约为680bp，通过与组蛋白核心颗粒的高亲和力而显示出核小体定位活性，并具有方位和位置依赖性。在此，我们报道了人类β-珠蛋白基因座控制区(β-LCR)DNase I高敏感部位2(HS2)的增强子活性可以被位于距离HS2两个核小体的弯曲DNA调节，并且弯曲DNA上的核小体作为关键的核小体调节附近的核小体相。当关键核小体与HS2之间的距离超过80bp时，导致核小体核小体结合部位偏离核小体二聚体轴线的红系特异性核小体相被过度表达。在这种状态下，增强子活性约为原始构建物中的50%，可能是由于转录因子结合减少所致。
A class of curved DNA appears universally in eukaryotic genomic DNA at an average distance of approximately 680 bp and shows nucleosome positioning activity by having high affinity for histone core particles in an orientation- and position-dependent manner. Here, we report that the enhancer activity at DNase I hypersensitive site 2 (HS2) of the human beta-globin locus control region (beta-LCR) can be modulated by the curved DNA located at a distance of two nucleosomes from HS2 and that the nucleosome at the curved DNA regulates nearby nucleosome phases as a key nucleosome. Erythroid-specific nucleosome phases which caused deviation of the NF-E2 (p18-p45 dimer) binding site from the nucleosome dyad axis were over-represented when the distance between the key nucleosome and HS2 exceeded 80 bp longer than the original length. At this state, enhancer activity was approximately 50% of that in the original construct, presumably due to reduced binding of transcription factors.
在转基因小鼠中使用微型构建体将人类 γ 球蛋白基因转换为 β 球蛋白。
影响因子：
5.3
作者：
Lloyd,JA;Krakowsky,JM;Crable,SC;Lingrel,JB
通讯作者：
Lingrel,JB
DOI：
10.1073/pnas.82.19.6384
发表时间：
1985-01-01
影响因子：
11.1
作者：
TUAN, D;SOLOMON, W;LONDON, IM
通讯作者：
LONDON, IM