Large-scale gene disruption using the UAU1 cassette.
Large-scale gene disruption using the UAU1 cassette.
复制标题
DOI:
10.1007/978-1-60327-151-6_17
复制
发表时间:
2009
期刊:
影响因子:
--
通讯作者:
Mitchell, Aaron P.
中科院分区:
文献类型:
--
作者:
Nobile, Clarissa J.;Mitchell, Aaron P.
Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.
影响因子:
5.3
作者:
Davis, D;Wilson, RB;Mitchell, AP
通讯作者:
Mitchell, AP
影响因子:
3.2
作者:
Wilson, RB;Davis, D;Mitchell, AP
通讯作者:
Mitchell, AP
影响因子:
3.2
作者:
Enloe, B;Diamond, A;Mitchell, AP
通讯作者:
Mitchell, AP