Large-scale gene disruption using the UAU1 cassette.

Large-scale gene disruption using the UAU1 cassette.
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DOI:
10.1007/978-1-60327-151-6_17
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发表时间:
2009
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Mitchell, Aaron P.
Mitchell, Aaron P.
中科院分区:
其他
文献类型:
--
作者:
Nobile, Clarissa J.;Mitchell, Aaron P.

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白色念珠菌是人类主要的真菌性系统性病原体。C.的遗传操作。白色念珠菌是不实用的。我们在这里报告的策略,是有用的和成功的大规模遗传操作的C。感兴趣的白色念珠菌基因:在Tn7转座子上使用UAU 1盒。简化但公认有缺陷的破坏技术,如这里所描述的,可能对揭示真菌毒力的遗传基础至关重要。
Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.
DOI: 10.1128/mcb.20.3.971-978.2000
发表时间: 2000-02-01
影响因子: 5.3
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发表时间: 1999-03-01
影响因子: 3.2
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影响因子: 3.2
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