Short-Term Administration of Common Anesthetics Does Not Dramatically Change the Endogenous Peptide Profile in the Rat Pituitary.
Short-Term Administration of Common Anesthetics Does Not Dramatically Change the Endogenous Peptide Profile in the Rat Pituitary.
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DOI：
10.1021/acschemneuro.2c00359
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发表时间：
2022-10-05
影响因子：
5
通讯作者：
Checco JW
中科院分区：
文献类型：
--
作者：
Mousavi S;Qiu H;Heinis FI;Abid MSR;Andrews MT;Checco JW
细胞间信号肽（例如肽激素、神经肽）是最大类别的细胞递质之一，调节多种生理过程。为了识别和量化不同生理状态下细胞间信号肽的相对丰度，基于 LC-MS 的肽组学工作流程通常用于新鲜解剖的组织。在此类动物实验中，全身麻醉是许多研究项目的重要一步。然而，急性麻醉可能会迅速改变递质分子和代谢物的测量丰度，特别是在大脑和内分泌系统中，这会混淆实验结果。本研究的目的是评估短期（< 5 分钟）麻醉给药对测量的细胞间信号肽丰度的影响，通过典型的肽组学工作流程进行评估。为了实现这一目标，我们比较了给予 5% 异氟烷、200 mg/kg 戊巴比妥钠或不给予麻醉剂后大鼠垂体中内源肽的丰度。无标记肽组学分析表明，急性使用异氟烷改变了少数肽的水平，主要是生长激素的降解产物，但不影响大多数其他肽激素的水平。紧急使用戊巴比妥钠对所有测量的肽的相对丰度的影响可以忽略不计。总体而言，我们的结果表明，垂体肽组学研究中使用的麻醉剂不会显着混淆观察到的结果。
Cell-cell signaling peptides (e.g., peptide hormones, neuropeptides) are among the largest class of cellular transmitters and regulate a variety of physiological processes. To identify and quantify the relative abundances of cell-cell signaling peptides in different physiological states, LC-MS-based peptidomics workflows are commonly utilized on freshly dissected tissue. In such animal experiments, the administration of general anesthetics is an important step for many research projects. However, acute anesthetic administration may rapidly change the measured abundance of transmitter molecules and metabolites, especially in the brain and endocrine system, which would confound experimental results. The aim of this study was to evaluate the effect of short-term (< 5 min) anesthetic administration on the measured abundance of cell-cell signaling peptides, as evaluated by a typical peptidomics workflow. To accomplish this goal, we compared endogenous peptide abundances in the rat pituitary following administration of 5% isoflurane, 200 mg/kg sodium pentobarbital, or no anesthetic administration. Label-free peptidomics analysis demonstrated that acute use of isoflurane changed the levels of a small number of peptides, primarily degradation products of the hormone somatotropin, but did not influence the levels of most other peptide hormones. Acute use of sodium pentobarbital had a negligible impact on the relative abundance of all measured peptides. Overall, our results suggest that anesthetics used in pituitary peptidomics studies do not dramatically confound observed results.
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小鼠脑和垂体中遗传定义的细胞类型的神经肽分析。
影响因子：
8.6
作者：
Fricker LD;Tashima AK;Fakira AK;Hochgeschwender U;Wetsel WC;Devi LA
通讯作者：
Devi LA
影响因子：
4.1
作者：
Dowd SE;Mustroph ML;Romanova EV;Southey BR;Pinardo H;Rhodes JS;Sweedler JV
通讯作者：
Sweedler JV
影响因子：
4.8
作者：
Che, FY;Yuan, Q;Fricker, LD
通讯作者：
Fricker, LD
影响因子：
5.7
作者：
FINCK, AD;SAMANIEGO, E;NGAI, SH
通讯作者：
NGAI, SH
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y