Exploring Exercise- and Context-Induced Peptide Changes in Mice by Quantitative Mass Spectrometry.
Exploring Exercise- and Context-Induced Peptide Changes in Mice by Quantitative Mass Spectrometry.
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DOI：
10.1021/acsomega.8b01713
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发表时间：
2018-10-31
期刊：
影响因子：
4.1
通讯作者：
Sweedler JV
中科院分区：
文献类型：
--
作者：
Dowd SE;Mustroph ML;Romanova EV;Southey BR;Pinardo H;Rhodes JS;Sweedler JV
最近的研究表明，运动可能有助于戒除可卡因成瘾，但其机制尚不清楚。在小鼠中，滚轮加速了可卡因条件性位置偏好（CPP）的消失，为评估潜在的神经机制提供了动物模型。本研究的目的是量化暴露于可卡因影响的小鼠杏仁核和海马齿状回内源肽的动态变化，以及对运动的反应。将可卡因调节的雄性 C57BL/6J 小鼠在有或没有跑轮的情况下圈养 30 天。在进行 CPP 测试并最终暴露于可卡因或盐水相关环境后，使用无标记基质辅助激光解吸/电离质谱 (MS) 以及使用液相色谱和电喷雾电离 MS 进行稳定同位素标记来测量脑组织提取物中的肽。跑步后小鼠的 CPP 显着降低，这与齿状回提取物中髓鞘碱性蛋白衍生物的减少相关，可能反映了无髓鞘颗粒神经元密度的增加。接触可卡因配对的环境会增加跑步者的血红蛋白衍生肽，并减少久坐动物的肌动蛋白衍生肽。这些结果使我们能够表征一组新颖的生物标志物，这些生物标志物对海马体中的运动和杏仁核中可卡因配对的环境有反应。
Recent research suggests that exercise may help facilitate abstinence from cocaine addiction, though the mechanisms are not well understood. In mice, wheel running accelerates the extinction of conditioned place preference (CPP) for cocaine, providing an animal model for evaluating potential neurological mechanisms. The objective of this study was to quantify dynamic changes in endogenous peptides in the amygdala and dentate gyrus of the hippocampus in mice exposed to a context paired with the effects of cocaine, and in response to exercise. Male C57BL/6J mice conditioned to cocaine were housed with or without running wheels for 30 days. Following a CPP test and final exposure to either a cocaine- or saline-associated context, peptides were measured in brain tissue extracts using label-free matrix-assisted laser desorption/ionization mass spectrometry (MS) and stable isotopic labeling with liquid chromatography and electrospray ionization MS. CPP in mice was significantly reduced with running, which correlated to decreased myelin basic protein derivatives in the dentate gyrus extracts, possibly reflecting increased unmyelinated granule neuron density. Exposure to a cocaine-paired context increased hemoglobin-derived peptides in runners and decreased an actin-derived peptide in sedentary animals. These results allowed us to characterize a novel set of biomarkers that are responsive to exercise in the hippocampus and in a cocaine-paired context in the amygdala.
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影响因子：
3.5
作者：
Griffin, Eadaoin W.;Bechara, Ranya G.;Kelly, Aine M.
通讯作者：
Kelly, Aine M.
影响因子：
--
作者：
Fricker LD
通讯作者：
Fricker LD
DOI：
10.1073/pnas.0813216106
发表时间：
2009-09-08
影响因子：
11.1
作者：
Biagioli, Marta;Pinto, Milena;Gustincich, Stefano
通讯作者：
Gustincich, Stefano
DOI：
10.1016/j.bbapap.2013.01.043
发表时间：
2013-11
影响因子：
3.2
作者：
Gelman, Julia S.;Dasgupta, Sayani;Berezniuk, Iryna;Fricker, Lloyd D.
通讯作者：
Fricker, Lloyd D.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y