RBM24 Mediates Lymph Node Metastasis and Epithelial-Mesenchymal Transition in Human Hypopharyngeal Squamous Cell Carcinoma by Regulating Twist1.
RBM24 Mediates Lymph Node Metastasis and Epithelial-Mesenchymal Transition in Human Hypopharyngeal Squamous Cell Carcinoma by Regulating Twist1.
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DOI：
10.1155/2022/1205353
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发表时间：
2022
影响因子：
--
通讯作者：
Hu, Guohua
中科院分区：
文献类型：
--
作者：
Liu, Yuhong;Pan, Min;Lu, Tao;Li, Yanshi;Yu, Dan;Wang, Zhihai;Hu, Guohua
尽管RBM 24（RNA结合基序24）的靶RNA调节作用，涉及多种癌症的蛋白质，其在HSCC中的作用仍不清楚。本研究旨在探讨RBM 24对HSCC的影响。 采用qRT-PCR、IHC和蛋白质印迹的组合来评估RBM 24的HSCC组织水平。采用殖民地形成和CCK-8测定法评价细胞增殖潜能，采用transwell法检测侵袭和转移潜能。通过划痕试验和EMT（上皮-间充质转化）通过采用qRT-PCR结合蛋白质印迹和IF（免疫荧光）评估FaDu细胞运动性。通过对腘淋巴结（淋巴结）的转移建模来研究RBM 24对HSCC的体内作用。 在显示淋巴结转移的HSCC患者中，注意到显著的RBM 24下调，低RBM 24水平与不良结局之间存在内在关联。敲低RBM 24促进细胞增殖、迁移和浸润，而过表达则导致相反的效果并抑制EMT。RBM 24对FaDu细胞迁移和侵袭的抑制作用被Twist 1过表达逆转。RBM 24在体内对HSCC中的肿瘤演变和LN转移的抑制作用也被验证。 RBM 24作为一种抑癌基因，通过调控Twist 1的表达，实现了对HSCC中LN转移和EMT的抑制。
Despite the target RNA regulatory action of RBM24 (RNA Binding Motif 24), a protein implicated in multiple carcinomas, its role in HSCC remains unclear. Our study probed to understand the effect of RBM24 on HSCC. A combination of qRT-PCR, IHC, and western blot was employed to assess the HSCC tissue level of RBM24. The colony formation and CCK-8 assays were performed to estimate cellular proliferative potential, whereas the transwell assay was conducted to examine invasive and metastatic potential. The FaDu cell motility was assessed via the scratch-wound assay and EMT (epithelial-mesenchymal transition) by adopting qRT-PCR in conjunction with western blot and IF (immunofluorescence). The in-vivo effect of RBM24 on HSCC was investigated through modeling metastasis to the popliteal LNs (lymph nodes). Among HSCC patients showing metastasis to LNs, prominent RBM24 downregulation was noted, with an intrinsic association between low RBM24 level and poor outcome. Knocking down RBM24 promoted cell multiplication, migration, and infiltration, while overexpression led to the opposite effects and inhibited the EMT. RBM24's suppressive action against the FaDu cell mobility and invasion was reversed by Twist1 overexpression. RBM24's suppressive actions against the tumor evolution and LN metastasis in HSCC in-vivo were also validated. As a carcinoma inhibitor gene, RBM24 regulates Twist1 to achieve LN metastasis and EMT suppression in HSCC.
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影响因子：
6.7
作者：
Choi, Ji-Hye;Kwon, So M.;Woo, Hyun G.
通讯作者：
Woo, Hyun G.
Twist1 在调节 FaDu 细胞化学敏感性中的作用和机制
影响因子：
3.4
作者：
Lu S;Yu L;Mu Y;Ma J;Tian J;Xu W;Wang H
通讯作者：
Wang H
影响因子：
7.4
作者：
Li, Yanshi;Pan, Min;Lu, Tao;Yu, Dan;Liu, Chuan;Wang, Zhihai;Hu, Guohua
通讯作者：
Hu, Guohua
影响因子：
2.1
作者：
Jin, Donghao;Hidaka, Kyoko;Morisaki, Takayuki
通讯作者：
Morisaki, Takayuki
DOI：
10.1083/jcb.200601018
发表时间：
2006-03-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JM;Dedhar S;Kalluri R;Thompson EW
通讯作者：
Thompson EW