Direct analysis of biodegradable chelating agents based on liquid chromatography/electrospray ionization mass spectrometry using a metal-free hydrophilic interaction liquid chromatographic column
Direct analysis of biodegradable chelating agents based on liquid chromatography/electrospray ionization mass spectrometry using a metal-free hydrophilic interaction liquid chromatographic column
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使用无金属亲水相互作用液相色谱柱，基于液相色谱/电喷雾电离质谱法直接分析可生物降解的螯合剂
DOI：
10.1007/s44211-022-00247-8
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发表时间：
2023
影响因子：
1.6
通讯作者：
Hiroshi Hasegawa
中科院分区：
文献类型：
--
作者：
Takaya Murakami;Ryoichi Wakata;Aya Mamorita;Asami S. Mashio;Kuo Hong Wong;Satoshi Chinaka;Hiroshi Hasegawa
近年来，可生物降解的氨基多羧酸螯合剂作为环境持久性螯合剂如乙二胺-N，N，N′，N′-四乙酸的替代物引起了人们的注意。然而，螯合剂的检测需要与金属络合或通过酯化试剂衍生化，并且使用当前可用的分析方法对其进行直接检测仍然是一个挑战。本文采用超高效液相色谱/电喷雾四极杆/飞行时间质谱法，对生物可降解螯合剂乙二胺-N，N '-二琥珀酸、3-羟基-2，2 ′-亚氨基二琥珀酸、甲基甘氨酸-N，N'-二乙酸和N，N-双（羧甲基）-L-谷氨酸进行了直接分析。采用无金属亲水作用液相色谱柱，成功地实现了满意的保留和分离，具有良好的峰形。标准曲线在1.0-50 μM范围内线性良好，相关系数大于0.9988。检测限为0.04 ~ 0.12 μM。该方法可用于实验室条件下生物降解和光降解实验中4种螯合剂的定量分析。该方法具有快速、灵敏、不需要复杂的前处理步骤等优点，有望对环境水样中螯合剂的实际分析做出重要贡献。
Recently, biodegradable aminopolycarboxylic acid chelating agents have attracted attention as an alternative to environmentally persistent chelating agents such as ethylenediamine-N,N,N′,N′-tetraacetic acid. However, the detection of chelating agents requires complexation with metals or derivatization by esterification reagents, and their direct detection using the currently available analytical methods still represents a challenge. Herein, we describe a direct analytical method for the biodegradable chelating agents ethylenediamine-N,N'-disuccinic acid, 3-hydroxy-2,2′-iminodisuccinic acid, methylglycine-N,N'-diacetic acid, andN,N-bis(carboxymethyl)-l-glutamic acid, via ultra-performance liquid chromatography/electrospray ionization quadrupole/time-of-flight mass spectrometry. Satisfactory retention and separation with a good peak shape were successfully achieved using a metal-free hydrophilic interaction liquid chromatographic column. The calibration curves showed good linearity in the range of 1.0–50 μM with correlation coefficients greater than 0.9988. The detection limits ranged from 0.04 to 0.12 μM. Furthermore, the developed method could be applied to the quantitative analysis of the four chelating agents in biodegradation and photodegradation experiments at the laboratory level. The proposed method, which offers the advantages of quickness, sensitivity, and requiring no complicated pretreatment steps, is expected to contribute significantly to the practical analysis of chelating agents in environmental water samples.Graphical abstract
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影响因子：
4.8
作者：
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DOI：
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发表时间：
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期刊：
Bioscience, biotechnology, and biochemistry
影响因子：
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影响因子：
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作者：
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影响因子：
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