OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis.
OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis.
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OsRAD51 在促进水稻减数分裂中的同源重组中发挥着至关重要的作用。
DOI：
10.3390/ijms23179906
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发表时间：
2022-08-31
影响因子：
5.6
通讯作者：
Yu, Hengxiu
中科院分区：
文献类型：
--
作者：
Liu, Xiaofei;Cao, Yiwei;Du, Guijie;Zhang, Chao;Xu, Meng;Cheng, Zhukuan;Shen, Yi;Yu, Hengxiu
Meiotic recombination plays a pivotal role in achieving accurate chromosomal segregation and increasing genetic diversity. In the homologous recombination pathway, the detailed mechanisms of how OsRAD51 and OsDMC1 work in rice meiosis remain to be explored. Here, we obtained different types of mutants for Osrad51a1, Osrad51a2, Osdmc1a, and Osdmc1b through CRISPR/Cas9. Both Osrad51a1 and Osrad51a2 exhibited normal vegetative growth and fertility. Osrad51 (Osrad51a1 Osrad51a2) mutant plants show normal vegetative growth but exhibit complete sterility, indicating that OsRAD51A1 and OsRAD51A2 are functionally redundant in rice fertility. In contrast to the wild type, Osrad51 chromosomes are not paired perfectly at pachytene and synaptonemal complex (SC) formation is deficient. Moreover, univalents and multivalent associations were observed at metaphase I, chromosome fragments presented at anaphase I, and crossover formation is basically suppressed in Osrad51 pollen mother cells (PMCs). OsRAD51 foci emerge at leptotene and disappear from late pachytene and chromosome localization of OsRAD51 depends on the formation of double-strand breaks (DSBs). Most OsRAD51 foci can co-localize with OsDMC1 signals. OsRAD51 is essential for the loading of OsDMC1 onto chromosomes, and vice versa. In addition, both OsRAD51 and OsDMC1 can interact with OsFIGL1 and OsBRCA2, two important components in rice meiosis. Moreover, the Osrad51 Osdmc1 (Osrad51a1 Osrad51a2 Osdmc1a Osdmc1b) quadruple mutant PMCs exhibited similar defective phenotypes as Osrad51 in homologous pairing, synapsis, and DSB repair. Taken together, our results suggest that the recombinases DMC1 and RAD51 may functionally depend on each other and play important roles in meiotic recombination during meiosis in rice.
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影响因子：
1.6
作者：
Dickey JS;Redon CE;Nakamura AJ;Baird BJ;Sedelnikova OA;Bonner WM
通讯作者：
Bonner WM
影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
2.6
作者：
Cuadrado, Angeles;Jouve, Nicolas
通讯作者：
Jouve, Nicolas
DOI：
10.1126/science.1219379
发表时间：
2012-09-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Cloud V;Chan YL;Grubb J;Budke B;Bishop DK
通讯作者：
Bishop DK
影响因子：
5.3
作者：
Li, Q;Lau, A;Stanley, EF
通讯作者：
Stanley, EF