Effect of Low-Dose Alcohol Consumption on Inflammation Following Transient Focal Cerebral Ischemia in Rats.
Effect of Low-Dose Alcohol Consumption on Inflammation Following Transient Focal Cerebral Ischemia in Rats.
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DOI：
10.1038/s41598-017-12720-w
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发表时间：
2017-10-02
影响因子：
4.6
通讯作者：
Sun H
中科院分区：
文献类型：
--
作者：
McCarter KD;Li C;Jiang Z;Lu W;Smith HA;Xu G;Mayhan WG;Sun H
越来越多的证据表明，低剂量饮酒（LAC）可降低缺血性卒中的发生率并改善功能结局。我们确定了LAC对缺血后炎症的影响。将雄性Sprague-Dawley大鼠分为3组，乙醇（13.5%酒精）组、红葡萄酒（城堡岩黑比诺，13.5%酒精）组和对照组。给予红酒和乙醇组的酒精量为1.4 g/kg/天。8周后，对动物进行2小时的大脑中动脉闭塞（MCAO），并在再灌注24小时时处死。评价脑缺血/再灌注（I/R）损伤、粘附分子和促炎和抗炎细胞因子/趋化因子的表达、小胶质细胞活化和中性粒细胞浸润。与对照组大鼠相比，红葡萄酒和乙醇喂养大鼠的总梗死体积和神经功能缺损显著减少。红葡萄酒和乙醇都抑制了缺血后粘附分子的表达和小胶质细胞的活化。此外，红葡萄酒和乙醇均上调金属蛋白酶组织抑制剂1（TIMP-1）的表达，下调促炎细胞因子/趋化因子的表达，并显著减轻缺血后炎症介质的表达。此外，红葡萄酒显着减少缺血后中性粒细胞浸润。我们的研究结果表明，LAC可能通过抑制缺血后炎症反应来保护脑免受I/R损伤。
Increasing evidence suggest that low-dose alcohol consumption (LAC) reduces the incidence and improves the functional outcome of ischemic stroke. We determined the influence of LAC on post-ischemic inflammation. Male Sprague-Dawley rats were divided into 3 groups, an ethanol (13.5% alcohol) group, a red wine (Castle Rock Pinot Noir, 13.5% alcohol) group, and a control group. The amount of alcohol given to red wine and ethanol groups was 1.4 g/kg/day. After 8 weeks, the animals were subjected to a 2-hour middle cerebral artery occlusion (MCAO) and sacrificed at 24 hours of reperfusion. Cerebral ischemia/reperfusion (I/R) injury, expression of adhesion molecules and pro- and anti-inflammatory cytokines/chemokines, microglial activation and neutrophil infiltration were evaluated. The total infarct volume and neurological deficits were significantly reduced in red wine- and ethanol-fed rats compared to control rats. Both red wine and ethanol suppressed post-ischemic expression of adhesion molecules and microglial activation. In addition, both red wine and ethanol upregulated expression of tissue inhibitor of metalloproteinases 1 (TIMP-1), downregulated expression of proinflammatory cytokines/chemokines, and significantly alleviated post-ischemic expression of inflammatory mediators. Furthermore, red wine significantly reduced post-ischemic neutrophil infiltration. Our findings suggest that LAC may protect the brain against its I/R injury by suppressing post-ischemic inflammation.
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影响因子：
--
作者：
Kang PF;Wu WJ;Tang Y;Xuan L;Guan SD;Tang B;Zhang H;Gao Q;Wang HJ
通讯作者：
Wang HJ
影响因子：
6.3
作者：
Justicia, C;Martín, A;Planas, AM
通讯作者：
Planas, AM
影响因子：
3.7
作者：
Sapkota A;Gaire BP;Cho KS;Jeon SJ;Kwon OW;Jang DS;Kim SY;Ryu JH;Choi JW
通讯作者：
Choi JW
DOI：
10.1136/bmj.d671
发表时间：
2011-02-22
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Ronksley PE;Brien SE;Turner BJ;Mukamal KJ;Ghali WA
通讯作者：
Ghali WA
影响因子：
37.8
作者：
Chu, Louis M.;Lassaletta, Antonio D.;Sellke, Frank W.
通讯作者：
Sellke, Frank W.