Revisiting Myosin Families Through Large-scale Sequence Searches Leads to the Discovery of New Myosins
Revisiting Myosin Families Through Large-scale Sequence Searches Leads to the Discovery of New Myosins
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通过大规模序列搜索重新审视肌球蛋白家族导致新肌球蛋白的发现
DOI：
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
R. Sowdhamini
中科院分区：
文献类型：
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作者：
S. N. Pasha;Iyer Meenakshi;R. Sowdhamini
肌球蛋白是肌动蛋白为基础的运动蛋白，参与许多细胞运动。研究各种类型肌球蛋白的进化模式和功能属性是有趣的。通过系统发育分析、序列基序和共存结构域，进行计算搜索算法以确定推定的肌球蛋白成员。本研究旨在了解各种分类群和可用真核基因组中编码的肌球蛋白的分布和可能的生物学功能。我们在这里报告的系统发育分析约4,064肌球蛋白马达域，完全从完整或接近完整的肌球蛋白库纳入许多未分类的，未表征的序列和新的肌球蛋白类，重点肌球蛋白从真菌，Haptophyta，和其他Strasiles，Alveolates，和Rhizaria（SAR）。在卵菌纲、细胞黏菌、Choanoflagellates、Pelagophytes、Eustigmatophyceae、Fonticula、Eucoccidiorida和Apicomplexans中发现了大类肌球蛋白，这些肌球蛋白具有保守的新型肌球蛋白基序变体，因此推测其功能扩展了我们对这个重要的马达蛋白家族的了解。这项工作提供了深入了解肌球蛋白的分布和可能的功能，包括新发现的肌球蛋白类。
Myosins are actin-based motor proteins involved in many cellular movements. It is interesting to study the evolutionary patterns and the functional attributes of various types of myosins. Computational search algorithms were performed to identify putative myosin members by phylogenetic analysis, sequence motifs, and coexisting domains. This study is aimed at understanding the distribution and the likely biological functions of myosins encoded in various taxa and available eukaryotic genomes. We report here a phylogenetic analysis of around 4,064 myosin motor domains, built entirely from complete or near-complete myosin repertoires incorporating many unclassified, uncharacterized sequences and new myosin classes, with emphasis on myosins from Fungi, Haptophyta, and other Stramenopiles, Alveolates, and Rhizaria (SAR). The identification of large classes of myosins in Oomycetes, Cellular slime molds, Choanoflagellates, Pelagophytes, Eustigmatophyceae, Fonticula, Eucoccidiorida, and Apicomplexans with novel myosin motif variants that are conserved and thus presumably functional extends our knowledge of this important family of motor proteins. This work provides insights into the distribution and probable function of myosins including newly identified myosin classes.
DOI：
10.1073/pnas.90.2.659
发表时间：
1993-01-15
影响因子：
11.1
作者：
GOODSON, HV;SPUDICH, JA
通讯作者：
SPUDICH, JA