The binding site for the liver-specific transcription factor Tf-LF1 and the TATA box of the human transferrin gene promoter are the only elements necessary to direct liver-specific transcription in vitro.
The binding site for the liver-specific transcription factor Tf-LF1 and the TATA box of the human transferrin gene promoter are the only elements necessary to direct liver-specific transcription in vitro.
复制标题
肝脏特异性转录因子 Tf-LF1 的结合位点和人转铁蛋白基因启动子的 TATA 盒是体外指导肝脏特异性转录所需的唯一元件。
作者：
D. Mendelzon;F. Boissier;M. Zakin
我们研究了人转铁蛋白基因启动子的肝脏特异性转录活性。竞争实验、定点突变和5'缺失分析的结果表明，TATA盒和肝特异性蛋白Tf-LF 1的结合位点是指导肝特异性体外转录所需的唯一元件。因此，Tf-LF 1的行为与其他先前描述的蛋白质HNF-1、DBP和LF-A1相同，因为它足以在体外赋予启动子肝脏特异性转录活性。这一结果与瞬时表达实验中的观察结果形成对比，在瞬时表达实验中，Tf-LF 1单独结合不能指导肝特异性表达，并且需要至少一种类似于C/EBP的蛋白质的结合。因此，如对其他肝基因所描述的，赋予组织特异性所需的元件的数量在体内和体外是不同的。
We have studied the liver-specific transcriptional activity of the human transferrin gene promoter. Results of competition experiments, site-directed mutagenesis, and 5' deletion analysis have demonstrated that a TATA box and a binding site for the liver-specific protein Tf-LF1 are the only elements needed to direct hepatic-specific transcription in vitro. Thus, Tf-LF1 behaves as other previously described proteins, HNF-1, DBP and LF-A1, in that it is sufficient to confer liver-specific transcriptional activity to a promoter in vitro. This results contrast with observations made in transient expression experiments, in which Tf-LF1 binding alone cannot direct hepatic-specific expression, and the binding of at least one more protein, similar to C/EBP, is needed. Thus, as described for other hepatic genes, the number of elements necessary to confer tissue specificity is different in vivo and in vitro.
影响因子：
56.9
作者：
COURTOIS, G;MORGAN, JG;CRABTREE, GR
通讯作者：
CRABTREE, GR
影响因子：
10.5
作者：
PINKERT, CA;ORNITZ, DM;PALMITER, RD
通讯作者：
PALMITER, RD