Food and water restriction lead to differential learning behaviors in a head-fixed two-choice visual discrimination task for mice.
Food and water restriction lead to differential learning behaviors in a head-fixed two-choice visual discrimination task for mice.
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DOI：
10.1371/journal.pone.0204066
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Hübener M
中科院分区：
文献类型：
--
作者：
Goltstein PM;Reinert S;Glas A;Bonhoeffer T;Hübener M
头部固定的行为任务可以提供重要的洞察啮齿动物的认知过程。尽管这种实验方法被广泛使用，但对任务参数（如动机激励）的差异如何影响整体任务绩效的了解有限。在这里，我们提供了一个详细的方法论描述的设置和程序，有效地训练小鼠的两个选择舔左/舔右视觉辨别任务。我们描述了两种不同的限制方案，即食物和水的限制，对动物的健康，活动模式，任务获取和性能的影响。虽然我们观察到在食物和水限制期间行为活动减少，但在整个实验中，无论限制方案如何，平均动物不适评分均保持在“亚阈值”和“轻度”类别。我们发现，限制的类型显着影响任务获取和参与的特定方面，即达到学习标准之前的会话数量和每次会话进行的试验数量，但它并不影响最大学习曲线性能。这些结果表明，限制范式的选择不会强烈影响动物的健康，但它可以对小鼠在任务中的表现产生显着影响。
Head-fixed behavioral tasks can provide important insights into cognitive processes in rodents. Despite the widespread use of this experimental approach, there is only limited knowledge of how differences in task parameters, such as motivational incentives, affect overall task performance. Here, we provide a detailed methodological description of the setup and procedures for training mice efficiently on a two-choice lick left/lick right visual discrimination task. We characterize the effects of two distinct restriction regimens, i.e. food and water restriction, on animal wellbeing, activity patterns, task acquisition, and performance. While we observed reduced behavioral activity during the period of food and water restriction, the average animal discomfort scores remained in the ‘sub-threshold’ and ‘mild’ categories throughout the experiment, irrespective of the restriction regimen. We found that the type of restriction significantly influenced specific aspects of task acquisition and engagement, i.e. the number of sessions until the learning criterion was reached and the number of trials performed per session, but it did not affect maximum learning curve performance. These results indicate that the choice of restriction paradigm does not strongly affect animal wellbeing, but it can have a significant effect on how mice perform in a task.
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影响因子：
8.8
作者：
Burgess CP;Lak A;Steinmetz NA;Zatka-Haas P;Bai Reddy C;Jacobs EAK;Linden JF;Paton JJ;Ranson A;Schröder S;Soares S;Wells MJ;Wool LE;Harris KD;Carandini M
通讯作者：
Carandini M
影响因子：
16.2
作者：
Aronov, Dmitriy;Tank, David W.
通讯作者：
Tank, David W.
影响因子：
5.3
作者：
Andermann ML;Kerlin AM;Reid RC
通讯作者：
Reid RC
DOI：
10.30802/aalas-jaalas-17-000122
发表时间：
2018-05-01
影响因子：
1.7
作者：
Giles, Jareca M.;Whitaker, Julia W.;Fletcher, Craig A.
通讯作者：
Fletcher, Craig A.
影响因子：
25
作者：
Mardinly AR;Oldenburg IA;Pégard NC;Sridharan S;Lyall EH;Chesnov K;Brohawn SG;Waller L;Adesnik H
通讯作者：
Adesnik H