The Organoid Era Permits the Development of New Applications to Study Glioblastoma.
The Organoid Era Permits the Development of New Applications to Study Glioblastoma.
复制标题
DOI：
10.3390/cancers12113303
复制
发表时间：
2020-11-09
期刊：
影响因子：
5.2
通讯作者：
Alaimo A
中科院分区：
文献类型：
--
作者：
Andreatta F;Beccaceci G;Fortuna N;Celotti M;De Felice D;Lorenzoni M;Foletto V;Genovesi S;Rubert J;Alaimo A
胶质母细胞瘤是最致命的成人原发脑肿瘤。胶质母细胞瘤涉及的大量突变和侵袭性使这种类型的癌症特别难以研究。为了解决这个问题，脑有机体已经成为研究脑生物学和概述胶质母细胞瘤发生的主要步骤的有前途的工具。本文综述了脑器官发育的方法，介绍了诱导胶质母细胞瘤的方法，以及直接从患者活检组织中获得胶质母细胞瘤器官的方法，并讨论了它们的局限性和未来的发展方向。胶质母细胞瘤(GB)是最常见和最具侵袭性的胶质瘤类型。缺乏可靠的GB模型，加上其相当大的临床异质性，阻碍了对导致肿瘤发生、癌症进展和治疗反应的机制的全面研究。最近，3D培养为克服这些挑战打开了可能性，脑有机体正在成为GB研究的前沿工具。通过基因编辑轻松设计脑器官并与患者来源的肿瘤球体进行共培养的机会使在更好地模拟脑组织结构的背景下分析癌症的发展成为可能。此外，从GB患者衍生的有机化合物中建立生物库是改进精准药物治疗的关键起点。这篇综述举例说明了胶质母细胞瘤3D模型的相关方面，特别关注有机物质及其在基础和翻译研究中的参与。
Glioblastoma is the most lethal primary adult brain tumor. The great number of mutations involved and the aggressiveness of glioblastoma render this type of cancer especially difficult to investigate. To address this problem, cerebral organoids have emerged as promising tools to investigate brain biology and to recapitulates the major steps involved in glioblastoma tumorigenesis. This review focuses on methods of cerebral organoid development, describes the protocols used for inducing glioblastoma, the approach used to derive glioblastoma organoids directly from patients’ biopsies and discusses their limitations and potential future direction. Glioblastoma (GB) is the most frequent and aggressive type of glioma. The lack of reliable GB models, together with its considerable clinical heterogeneity, has impaired a comprehensive investigation of the mechanisms that lead to tumorigenesis, cancer progression, and response to treatments. Recently, 3D cultures have opened the possibility to overcome these challenges and cerebral organoids are emerging as a leading-edge tool in GB research. The opportunity to easily engineer brain organoids via gene editing and to perform co-cultures with patient-derived tumor spheroids has enabled the analysis of cancer development in a context that better mimics brain tissue architecture. Moreover, the establishment of biobanks from GB patient-derived organoids represents a crucial starting point to improve precision medicine therapies. This review exemplifies relevant aspects of 3D models of glioblastoma, with a specific focus on organoids and their involvement in basic and translational research.
登录
查看更多内容
影响因子：
23.9
作者：
Bershteyn M;Nowakowski TJ;Pollen AA;Di Lullo E;Nene A;Wynshaw-Boris A;Kriegstein AR
通讯作者：
Kriegstein AR
影响因子：
48
作者：
Bian S;Repic M;Guo Z;Kavirayani A;Burkard T;Bagley JA;Krauditsch C;Knoblich JA
通讯作者：
Knoblich JA
影响因子：
64.5
作者：
Gao D;Vela I;Sboner A;Iaquinta PJ;Karthaus WR;Gopalan A;Dowling C;Wanjala JN;Undvall EA;Arora VK;Wongvipat J;Kossai M;Ramazanoglu S;Barboza LP;Di W;Cao Z;Zhang QF;Sirota I;Ran L;MacDonald TY;Beltran H;Mosquera JM;Touijer KA;Scardino PT;Laudone VP;Curtis KR;Rathkopf DE;Morris MJ;Danila DC;Slovin SF;Solomon SB;Eastham JA;Chi P;Carver B;Rubin MA;Scher HI;Clevers H;Sawyers CL;Chen Y
通讯作者：
Chen Y
影响因子：
11.2
作者：
Galli, R;Binda, E;Vescovi, A
通讯作者：
Vescovi, A
影响因子：
3
作者：
Al-Mayhani, Talal M. Fael;Ball, Siolian L. R.;Watts, Colin
通讯作者：
Watts, Colin