miR-185 inhibits cell migration and invasion of hepatocellular carcinoma through CDC42.
miR-185 inhibits cell migration and invasion of hepatocellular carcinoma through CDC42.
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DOI：
10.3892/ol.2018.8971
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发表时间：
2018-09
期刊：
影响因子：
2.9
通讯作者：
Liu K
中科院分区：
文献类型：
--
作者：
Zhang Q;Chen Y;Liu K
肝细胞癌（HCC）是一种发病率高、死亡率高的原发性肝癌。miR-185是一种大约有22-28个核苷酸的microRNA，据报道与许多癌症有关。研究了miR-185通过细胞分裂周期42 （CDC42）作用于HCC的潜在机制。RT-qPCR检测HCC组织和细胞中miR-185和CDC42的RNA水平。采用双荧光素酶报告基因试验验证CDC42是否是miR-185的靶基因。Transwell法检测迁移和侵袭对miR-185的影响。miR-185在HCC中的表达较低，且与CDC42呈负相关。miR-185抑制HCC的迁移、侵袭，miR-185低表达预示预后不良。CDC42被预测为miR-185的靶基因，并受miR-185调控。miR-185通过CDC42抑制细胞在HCC中的迁移和侵袭能力。综上所述，miR-185通过直接靶向CDC42抑制HCC细胞的迁移和侵袭。这表明miR-185/CDC42轴可能是HCC治疗的新靶点。
Hepatocellular carcinoma (HCC) is a primary liver cancer with high incidence and mortality. miR-185, a microRNA with appriximately 22-28 nucleotides, was reported to be involved in many cancers. The potential mechanism of miR-185 on HCC through cell division cycle 42 (CDC42) was investigated. RT-qPCR was used to measure the RNA level of miR-185 and CDC42 in HCC tissues and cells. The dual luciferase reporter assay was used to verify whether CDC42 was a target gene for miR-185. Transwell assay was employed to detect the ability of migration and invasion to change miR-185. miR-185 expression was low in HCC and negatively correlated with CDC42. miR-185 inhibited HCC migration, invasion and miR-185 low expression predicted poor prognosis. CDC42 was predicted to be a target gene for miR-185, and regulated by miR-185. miR-185 suspressed the ability of cell migration and invasion through CDC42 in HCC. In conclusion, miR-185 suspressed migration and invasion of HCC cells by directly targeting CDC42. It is suggested that miR-185/CDC42 axis may present a novel target for HCC treatment.
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