Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp. phaseoli.
Differentially Expressed Genes in Resistant and Susceptible Common Bean (Phaseolus vulgaris L.) Genotypes in Response to Fusarium oxysporum f. sp. phaseoli.
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抗性和感病菜豆 (Phaseolus vulgaris L.) 基因型中对尖孢镰刀菌 (Fusarium oxysporum f) 反应的差异表达基因。
DOI：
10.1371/journal.pone.0127698
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Blair MW
中科院分区：
文献类型：
--
作者：
Xue R;Wu J;Zhu Z;Wang L;Wang X;Wang S;Blair MW
菜豆枯萎病，由尖孢镰刀菌（Fusarium oxysporum Schlechtend）引起：菜豆白粉病是世界范围内菜豆的重要病害之一。对Fop的抗性的天然来源很少存在，并且仅提供中等或部分水平的保护。尽管该疾病在多种作物中具有经济重要性，但在豆类中仅分析了少数Fop诱导的基因。因此，我们的目标是确定转录调控基因之间的不相容的相互作用，菜豆和Fop病原体使用cDNA扩增片段长度多态性（cDNA-AFLP）技术。基于对中度抗性和敏感基因型的比较，我们用768个引物对产生了总共8，730个转录衍生片段（TDFs）。总共有423个TDFs（4.9%）在接种Fop接种物后显示出改变的表达模式。我们获得了122个选定的TDFs的完整扩增子序列，其中98个被确定为注释的已知基因在不同的功能类别的基础上，其推定的功能，10个预测，但非注释的基因和14个不同源的任何已知的基因。已知推定功能的98个TDFs编码基因被分类为与代谢（22）、信号转导（21）、蛋白质合成和加工（20）、发育和细胞骨架组织（12）、蛋白质转运（7）、基因表达和RNA代谢（4）、氧化还原反应（4）、防御和应激反应（3）、能量代谢（3）、和激素反应（2）。在同源性分析的基础上，选择了19个不同功能类别的TDFs进行定量RT-PCR表达分析。在此发现的重要基因与病原体感染的各个步骤有关，这将使我们更好地了解Fop和类似病原体的防御和抗性机制。所发现的差异反应基因也可作为分子标记用于关联作图或QTL分析。
Fusarium wilt of common bean (Phaseolus vulgaris L.), caused by Fusarium oxysporum Schlechtend.:Fr. f.sp. phaseoli (Fop), is one of the most important diseases of common beans worldwide. Few natural sources of resistance to Fop exist and provide only moderate or partial levels of protection. Despite the economic importance of the disease across multiple crops, only a few of Fop induced genes have been analyzed in legumes. Therefore, our goal was to identify transcriptionally regulated genes during an incompatible interaction between common bean and the Fop pathogen using the cDNA amplified fragment length polymorphism (cDNA-AFLP) technique. We generated a total of 8,730 transcript-derived fragments (TDFs) with 768 primer pairs based on the comparison of a moderately resistant and a susceptible genotype. In total, 423 TDFs (4.9%) displayed altered expression patterns after inoculation with Fop inoculum. We obtained full amplicon sequences for 122 selected TDFs, of which 98 were identified as annotated known genes in different functional categories based on their putative functions, 10 were predicted but non-annotated genes and 14 were not homologous to any known genes. The 98 TDFs encoding genes of known putative function were classified as related to metabolism (22), signal transduction (21), protein synthesis and processing (20), development and cytoskeletal organization (12), transport of proteins (7), gene expression and RNA metabolism (4), redox reactions (4), defense and stress responses (3), energy metabolism (3), and hormone responses (2). Based on the analyses of homology, 19 TDFs from different functional categories were chosen for expression analysis using quantitative RT-PCR. The genes found to be important here were implicated at various steps of pathogen infection and will allow a better understanding of the mechanisms of defense and resistance to Fop and similar pathogens. The differential response genes discovered here could also be used as molecular markers in association mapping or QTL analysis.
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影响因子：
3.1
作者：
Cho, YG;Blair, MW;McCouch, SR
通讯作者：
McCouch, SR
DOI：
10.1073/pnas.1016981108
发表时间：
2011-05-10
影响因子：
11.1
作者：
Cao, Aizhong;Xing, Liping;Chen, Peidu
通讯作者：
Chen, Peidu
影响因子：
4.4
作者：
Córdoba JM;Chavarro C;Schlueter JA;Jackson SA;Blair MW
通讯作者：
Blair MW
影响因子：
7.2
作者：
Bachem, CWB;vanderHoeven, RS;Visser, RGF
通讯作者：
Visser, RGF
影响因子：
4.2
作者：
Cordoba, Juana M.;Chavarro, Carolina;Blair, Matthew W.
通讯作者：
Blair, Matthew W.