Negative pressure wound therapy reduces the motility of Pseudomonas aeruginosa and enhances wound healing in a rabbit ear biofilm infection model.
Negative pressure wound therapy reduces the motility of Pseudomonas aeruginosa and enhances wound healing in a rabbit ear biofilm infection model.
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负压伤口治疗可降低兔耳生物膜感染模型中铜绿假单胞菌的活力并促进伤口愈合
DOI：
10.1007/s10482-018-1045-5
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Peifu T
中科院分区：
文献类型：
--
作者：
Guoqi W;Zhirui L;Song W;Tongtong L;Lihai Z;Licheng Z;Peifu T
Pseudomonas aeruginosa motility, virulence factors and biofilms are known to be detrimental to wound healing. The efficacy of negative pressure wound therapy (NPWT) against P. aeruginosa has been little studied, either in vitro or in vivo. The present study evaluated the effect of negative pressure (NP) on P. aeruginosa motility in vitro, and the effect of NPWT on virulence factors and biofilms in vivo. P. aeruginosa motility was quantified under different levels of NP (atmospheric pressure, - 75, - 125, - 200 mmHg) using an in vitro model. Swimming, swarming and twitching motility were significantly inhibited by NP (- 125 and - 200 mmHg) compared with atmospheric pressure (p = 0.05). Virulence factors and biofilm components were quantified in NPWT and gauze treated groups using a rabbit ear biofilm model. Biofilm structure was studied with fluorescence microscopy and scanning electron microscopy. Additionally, viable bacterial counts and histological wound healing parameters were measured. Compared with the control, NPWT treatment resulted in a significant reduction in expression of all virulence factors assayed including exotoxin A, rhamnolipid and elastase (p = 0.01). A significant reduction of biofilm components (eDNA) (p = 0.01) was also observed in the NPWT group. The reduction of biofilm matrix was verified by fluorescence- and scanning electron-microscopy. NPWT lead to better histologic parameters (p = 0.01) and decreased bacterial counts (p = 0.05) compared with the control. NPWT treatment was demonstrated to be an effective strategy to reduce virulence factors and biofilm components, which may explain the increased wound healing observed.
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影响因子：
3.7
作者：
Akers KS;Mende K;Cheatle KA;Zera WC;Yu X;Beckius ML;Aggarwal D;Li P;Sanchez CJ;Wenke JC;Weintrob AC;Tribble DR;Murray CK;Infectious Disease Clinical Research Program Trauma Infectious Disease Outcomes Study Group
通讯作者：
Infectious Disease Clinical Research Program Trauma Infectious Disease Outcomes Study Group
DOI：
10.1097/won.0b013e31827e8481
发表时间：
2013-03-01
影响因子：
2.6
作者：
Bradley, Beth Hawkins;Cunningham, Muriel
通讯作者：
Cunningham, Muriel
影响因子：
3.7
作者：
Liu Y;Zhou Q;Wang Y;Liu Z;Dong M;Wang Y;Li X;Hu D
通讯作者：
Hu D
DOI：
10.1097/00004630-199209000-00003
发表时间：
1992-01-01
期刊：
Journal of Burn Care and Rehabilitation
影响因子：
--
作者：
Heggers, John P.;Haydon, Scott;Robson, Martin C.
通讯作者：
Robson, Martin C.
影响因子：
2.3
作者：
Blum, Martin L.;Esser, Max;Rosenfeldt, Franklin L.
通讯作者：
Rosenfeldt, Franklin L.