MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells.
MicroRNA-27b inhibits Spry2 expression and promotes cell invasion in glioma U251 cells.
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DOI：
10.3892/ol.2015.2865
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发表时间：
2015-03
期刊：
影响因子：
2.9
通讯作者：
Fu J
中科院分区：
文献类型：
--
作者：
Liu C;Liang S;Xiao S;Lin Q;Chen X;Wu Y;Fu J
MicroRNA（miR）-27 b已被报道参与胶质瘤的形成。然而，miR-27 b的详细作用和潜在机制在很大程度上仍然未知。本研究发现miR-27 b在胶质瘤组织中的表达明显高于正常癌旁组织。此外，与正常人星形胶质细胞相比，miR-27 b在U87、U251和SHG 44胶质瘤细胞系中也上调。Sprouty homolog 2（Spry 2）是miR-27 b在U251胶质瘤细胞中的新靶点，与胶质瘤的侵袭性相关，并且在U251胶质瘤细胞中，Spry 2的蛋白表达受miR-27 b的负调控。此外，miR-27 b的抑制和Spry 2的上调抑制了胶质瘤细胞的侵袭，而Spry 2的下调逆转了miR-27 b抑制对胶质瘤细胞侵袭的抑制作用。这些数据表明，miR-27 b可能通过直接抑制Spry 2表达来促进胶质瘤细胞的侵袭。这些数据也表明miR-27 b可能成为抑制胶质瘤侵袭和转移的一个有希望的分子靶点。
MicroRNA (miR)-27b has been reported to participate in glioma. However, a detailed role of miR-27b and the underlying mechanism remain largely unknown. The present study found that the expression of miR-27b was significantly increased in glioma tissues compared with normal adjacent tissues. In addition, miR-27b was also upregulated in the U87, U251 and SHG44 glioma cell lines compared with normal human astrocytes. Sprouty homolog 2 (Spry2), which has been reported to be associated with invasive glioma, was identified as a novel target of miR-27b in U251 glioma cells, and the protein expression of Spry2 was negatively regulated by miR-27b in U251 cells. Additionally, inhibition of miR-27b and upregulation of Spry2 suppressed glioma cell invasion, while downregulation of Spry2 reversed the suppressive effect of miR-27b inhibition on glioma cell invasion. These data suggest that miR-27b may promote glioma cell invasion through direct inhibition of Spry2 expression. The data also suggest that miR-27b may become a promising molecular target for inhibiting the invasion and metastasis of glioma.
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影响因子：
2.1
作者：
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通讯作者：
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影响因子：
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影响因子：
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影响因子：
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作者：
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