A gold nanoparticle system for the enhancement of radiotherapy and simultaneous monitoring of reactive-oxygen-species formation.
A gold nanoparticle system for the enhancement of radiotherapy and simultaneous monitoring of reactive-oxygen-species formation.
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DOI：
10.1088/1361-6528/aae272
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发表时间：
2018-12-14
期刊：
影响因子：
3.5
通讯作者：
Pratx G
中科院分区：
文献类型：
--
作者：
Choi BJ;Jung KO;Graves EE;Pratx G
Gold nanoparticles (AuNPs) are known to sensitize cancer cells to radiation therapy (RT) by increasing the deposition of ionizing energy in their immediate vicinity. However, this process of dose enhancement is challenging to monitor because it is heterogeneous at the sub-cellular scale. Furthermore, radiation damage is primarily mediated by reactive oxygen species (ROS) that are produced following water radiolysis. Here, radiation-responsive PEGylated gold nanoparticles (RPAuNPs) were synthesized for enhanced generation and concurrent detection of ROS in cancer cells and tumors. PEGylated gold particles (20 nm diameter) were functionalized with dihydrorhodamine 123 (DHR-123), a known ROS sensor, to monitor ROS generation in their immediate vicinity. These nanoparticles were able to effectively radiosensitize cells, as measured by increased cell apoptosis following RT. Furthermore, the fluorescence of these RPAuNPs was sevenfold higher after 6 Gy RT due to local production of ROS near the surface of the nanoparticle. Finally, multispectral fluorescence imaging was used to monitor nanoparticle-induced ROS in vivo, following conformal RT, in a xenograft model of breast cancer. This theranostic nanoparticle system provides a novel approach for monitoring the nanoscale enhancement of RT by high-Z metal nanoparticles.
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DOI：
10.1038/nrclinonc.2015.120
发表时间：
2015-09
期刊：
Nature reviews. Clinical oncology
影响因子：
--
作者：
Schaue D;McBride WH
通讯作者：
McBride WH
影响因子：
5.7
作者：
McMahon, Stephen J.;Hyland, Wendy B.;Currell, Fred J.
通讯作者：
Currell, Fred J.
影响因子：
17.1
作者：
Cheng, Kai;Sano, Michael;Xing, Lei
通讯作者：
Xing, Lei
影响因子：
13.3
作者：
Blanch, Adam J.;Doeblinger, Markus;Rodriguez-Fernandez, Jessica
通讯作者：
Rodriguez-Fernandez, Jessica
影响因子：
--
作者：
Carrillo-Cazares, A.;Jimenez-Mancilla, N. P.;Camacho-Lopez, M. A.
通讯作者：
Camacho-Lopez, M. A.