Monitoring inflammation injuries in the progression of atherosclerosis with contrast enhanced ultrasound molecular imaging.
Monitoring inflammation injuries in the progression of atherosclerosis with contrast enhanced ultrasound molecular imaging.
复制标题
利用对比增强超声分子成像监测动脉粥样硬化进展中的炎症损伤
DOI：
10.1371/journal.pone.0186155
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Sun R;Tian J;Zhang J;Wang L;Guo J;Liu Y
The upregulation of vascular cell adhesion molecule-1(VCAM-1) on vascular endothelium plays a great role in the progression of atherosclerosis (AS). In this study, ultrasound molecular imaging was performed to monitor the inflammation injuries in the onset and progression of atherosclerosis with microbubbles targeted to VCAM-1. Mice deficient for the apolipoprotein E (ApoE-/-mice) with high-cholesterol diet were studied as an age-dependent model of atherosclerosis. At 8, 16, 24, and 32 weeks of age, contrast enhanced ultrasound (CEU) molecular imaging of proximal ascending aorta was performed with microbubbles targeted to VCAM-1. Plaque size, monocytes infiltration and the expression of VCAM-1 in the proximal ascending aorta were assessed by histology and western blot analysis, separately. In ApoE-/- mice, molecular imaging for VCAM-1 detected selective signal enhancement (P<0.01 versus non-targeted microbubbles) at all ages of ApoE-/- mice. Moreover, signals from targeted microbubbles increased from 8wks to 32wks age (P<0.05 for trend) in ApoE-/- mice, indicating the upregulation of VCAM-1 with the progression of atherosclerosis. Consistent with CEU imaging results, both western blot analysis and immunohistochemistry revealed the expression of VCAM-1 and monocytes infiltration were age-dependent in ApoE-/- mice. CEU molecular imaging can be used to noninvasively detect the VCAM-1 expression on the endothelium in the progression of atherosclerosis. By investigating specific molecular biomarkers, it could help to monitor the inflammation and the progression of AS, which may in some extent contribute to the prediction of vulnerable plaque.
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NADPH 氧化酶抑制抗氧化治疗对晚期动脉粥样硬化的炎症和血小板粘附作用的分子成像
DOI：
10.1161/circimaging.112.975193
发表时间：
2013-01-01
期刊：
Circulation. Cardiovascular imaging
影响因子：
--
作者：
Liu Y;Davidson BP;Yue Q;Belcik T;Xie A;Inaba Y;McCarty OJ;Tormoen GW;Zhao Y;Ruggeri ZM;Kaufmann BA;Lindner JR
通讯作者：
Lindner JR
影响因子：
20.1
作者：
Huo, YQ;Hafezi-Moghadam, A;Ley, K
通讯作者：
Ley, K
影响因子：
3.1
作者：
Zhang, Ming-Duo;Zhao, Xue-Cheng;Zhao, Quan-Ming
通讯作者：
Zhao, Quan-Ming
DOI：
10.1161/01.atv.14.1.133
发表时间：
1994-01-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
NAKASHIMA, Y;PLUMP, AS;ROSS, R
通讯作者：
ROSS, R
DOI：
10.1016/j.echo.2014.03.013
发表时间：
2014-07
影响因子：
6.5
作者：
Davidson, Brian P.;Chadderdon, Scott M.;Belcik, J. Todd;Gupta, Saurabh;Lindner, Jonathan R.
通讯作者：
Lindner, Jonathan R.