Ischemic memory imaging in nonhuman primates with echocardiographic molecular imaging of selectin expression.
Ischemic memory imaging in nonhuman primates with echocardiographic molecular imaging of selectin expression.
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DOI：
10.1016/j.echo.2014.03.013
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发表时间：
2014-07
影响因子：
6.5
通讯作者：
Lindner, Jonathan R.
中科院分区：
文献类型：
--
作者：
Davidson, Brian P.;Chadderdon, Scott M.;Belcik, J. Todd;Gupta, Saurabh;Lindner, Jonathan R.
选择素是在激活时由血管内皮表达的粘附分子，并且可以是用于在解决后长时间检测心肌缺血的成像靶标。我们假设选择素的分子成像与心肌声学造影（MCE）分子成像可用于检测最近短暂的缺血在闭胸非人灵长类动物。通过经皮球囊导管闭塞LAD或回旋冠状动脉5-10分钟，在麻醉的成年恒河猴（n=6）中产生心肌缺血。三只独立的猕猴作为非缺血对照。在冠状动脉闭塞期间进行MCE灌注成像以测量风险面积（RA），并在100-110 min进行MCE灌注成像以排除梗死。再灌注后30和90分钟，使用脂质微泡携带二聚体重组人P-选择素糖蛋白配体-1（MB-YSPSL）进行MCE分子成像。在基线、每次MB-YSPSL注射前和注射后10分钟采集用于安全性数据的血液、心电图和超声心动图。静脉注射MB-YSPSL后，生命体征、氧饱和度、ECG、心室收缩功能、肺血管阻力和血清安全性标志物均无变化。超声心动图显示，RA患者的LV功能障碍在30分钟内消退，MCE灌注成像显示无梗死证据。在选择素靶向MCE分子成像中，缺血后30 min（25±11 vs 11±4 IU）和90 min（13±3 vs 3±2 IU）RA的信号增强大于远端区域（p<0.05）。在对照非缺血受试者中没有增强（<1 IU）。在灵长类动物中，使用适合于人类的重组配体进行选择素的MCE分子成像对于近期心肌缺血成像既安全又有效。这项技术可能是有用的检测近期缺血患者胸痛，即使在没有坏死。
Selectins are adhesion molecules that are expressed by the vascular endothelium upon activation and may be an imaging target for detecting myocardial ischemia long after resolution. We hypothesized that molecular imaging of selectins with myocardial contrast echocardiography (MCE) molecular imaging could be used to detect recent brief ischemia in closed-chest non-human primates. Myocardial ischemia was produced in anesthetized adult rhesus macaques (n=6) by percutaneous balloon catheter occlusion of the LAD or circumflex coronary artery for 5–10 minutes. Three separate macaques served as non-ischemic controls. MCE perfusion imaging was performed during coronary occlusion to measure risk area (RA) and at 100–110 min to exclude infarction. MCE molecular imaging was performed at 30 and 90 min after reperfusion using a lipid microbubble bearing dimeric recombinant human P-selectin glycoprotein ligand-1 (MB-YSPSL). Collection of blood for a safety data, electrocardiography, and echocardiography were performed at baseline, and before and 10 min after each MB-YSPSL injection. Vital signs, O2 saturation, ECG, ventricular systolic function, pulmonary vascular resistance, and serum safety markers were unchanged by intravenous injection of MB-YSPSL. On echocardiography, LV dysfunction in the RA had resolved by 30 min and there was no evidence for infarction on MCE perfusion imaging. On selectin-targeted MCE molecular imaging, signal enhancement was greater (p<0.05) in the RA than remote territory at 30 min (25±11 vs 11±4 IU) and 90 min (13±3 vs 3±2 IU) after ischemia. There was no enhancement (<1 IU) in control non-ischemic subjects. In primates, MCE molecular imaging of selectins using a recombinant ligand appropriate for humans is both safe and effective for imaging recent myocardial ischemia. This technique may be useful for detecting recent ischemia in patients with chest pain even in the absence of necrosis.
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影响因子：
37.8
作者：
Chadderdon SM;Belcik JT;Bader L;Kirigiti MA;Peters DM;Kievit P;Grove KL;Lindner JR
通讯作者：
Lindner JR
影响因子：
24
作者：
Davidson, Brian P.;Kaufmann, Beat A.;Belcik, J. Todd;Xie, Aris;Qi, Yue;Lindner, Jonathan R.
通讯作者：
Lindner, Jonathan R.
影响因子：
3.2
作者：
Thomas, Regi;Cheng, Yulan;Nunn, Adrian D.
通讯作者：
Nunn, Adrian D.
超声心动图评估干细胞疗法对缺血性心肌病的灌注和功能的影响。
DOI：
10.1016/j.echo.2013.10.011
发表时间：
2014-02
影响因子：
6.5
作者：
Inaba, Yoichi;Davidson, Brian P.;Kim, Sajeevani;Liu, Ya Ni;Packwood, William;Belcik, J. Todd;Xie, Aris;Lindner, Jonathan R.
通讯作者：
Lindner, Jonathan R.
DOI：
10.1016/s0735-1097(98)00212-5
发表时间：
1998-07-01
影响因子：
24
作者：
Wei, K;Jayaweera, AR;Kaul, S
通讯作者：
Kaul, S