Induction and suppression of allergic diarrhea and systemic anaphylaxis in a murine model of food allergy.
Induction and suppression of allergic diarrhea and systemic anaphylaxis in a murine model of food allergy.
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DOI：
10.1016/j.jaci.2012.03.004
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Finkelman FD
中科院分区：
文献类型：
--
作者：
Kucuk ZY;Strait R;Khodoun MV;Mahler A;Hogan S;Finkelman FD
食物过敏的临床表现包括腹泻和全身性过敏反应（休克），这两种反应可以在不同的个体中同时发生或单独发生。虽然摄入的食物抗原需要被吸收以诱导休克，但尚不清楚它们是否需要被吸收以诱导腹泻。确定确定食物过敏是否诱发腹泻与休克的机制，并确定腹泻是否需要吸收摄入的抗原。方法：采用主动免疫、被动免疫和混合免疫小鼠模型研究这些问题。活性模型用于确定不能分泌伊加的J链和pIgR缺陷小鼠的过敏性腹泻易感性。杂交模型用于确定静脉内施用的抗原特异性IgG抗体（其不分泌到肠道中）是否可以防止过敏性腹泻以及休克。休克，但不是腹泻诱导在未经处理的小鼠静脉注射IgE抗TNP抗体，然后口服TNP-牛血清白蛋白，而这两种诱导小鼠腹腔内卵清蛋白/明矾加口服卵清蛋白预致敏。需要更多的TNP-牛血清白蛋白诱导休克比在致敏小鼠和静脉注射IgG抗TNP抗体，这是不分泌到肠道，保护这些小鼠对腹泻和休克。与此一致，OVA免疫的J链和pIgR缺陷小鼠，具有高血清伊加，但肠道伊加很少，抵抗腹泻诱导。肠道免疫和口服Ag剂量决定是否诱导腹泻和/或全身过敏反应，并且摄入的Ag必须被吸收以诱导任何一种反应。
The clinical manifestations of food allergy include diarrhea and systemic anaphylaxis (shock), which can occur together or by themselves in different individuals. Although ingested food antigens need to be absorbed to induce shock, it is not known whether they need to be absorbed to induce diarrhea. Identify mechanisms that determine whether food allergy induces diarrhea versus shock and determine whether diarrhea requires absorption of ingested antigens. Methods: These issues were studied in mice in active, passive and hybrid immunization models. The active model was used to determine the allergic diarrhea susceptibility of J chain- and pIgR-deficient mice, which are unable to secrete IgA. The hybrid model was used to determine whether intravenously administered antigen-specific IgG antibody, which is not secreted into the gut, can protect against allergic diarrhea as well as shock. Shock, but not diarrhea was induced in naïve mice by intravenous IgE anti-TNP antibody, followed by oral TNP-bovine serum albumin, whereas both were induced in mice presensitized with intraperitoneal ovalbumin/alum plus oral ovalbumin. More TNP-bovine serum albumin was required to induce shock than diarrhea in presensitized mice and intravenous IgG anti-TNP antibody, which is not secreted into the gut, protected these mice against both diarrhea and shock. Consistent with this, OVA-immunized J chain- and pIgR-deficient mice, which have high serum IgA but little intestinal IgA, resisted diarrhea induction. Intestinal immunity and oral Ag dose determine whether diarrhea and/or systemic anaphylaxis are induced and ingested Ag must be absorbed to induce either response.
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在聚合物免疫球蛋白受体/分泌成分缺陷的小鼠中，缺乏上皮免疫球蛋白A转运，粘膜泄漏增加。
影响因子：
15.3
作者：
Johansen, F E;Pekna, M;Norderhaug, I N;Haneberg, B;Hietala, M A;Krajci, P;Betsholtz, C;Brandtzaeg, P
通讯作者：
Brandtzaeg, P
影响因子：
14.2
作者：
Sicherer, Scott H.
通讯作者：
Sicherer, Scott H.
影响因子：
4.4
作者：
Strait, RT;Morris, SC;Finkelman, FD
通讯作者：
Finkelman, FD
影响因子：
14.2
作者：
Li, XM;Schofield, BH;Sampson, HA
通讯作者：
Sampson, HA
DOI：
10.1016/j.jaci.2010.12.1098
发表时间：
2011-03
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Nowak-Węgrzyn A;Sampson HA
通讯作者：
Sampson HA