Lactobacillus gasseri SBT2055 induces TGF-β expression in dendritic cells and activates TLR2 signal to produce IgA in the small intestine.
Lactobacillus gasseri SBT2055 induces TGF-β expression in dendritic cells and activates TLR2 signal to produce IgA in the small intestine.
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DOI：
10.1371/journal.pone.0105370
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Miyazaki T
中科院分区：
文献类型：
--
作者：
Sakai F;Hosoya T;Ono-Ohmachi A;Ukibe K;Ogawa A;Moriya T;Kadooka Y;Shiozaki T;Nakagawa H;Nakayama Y;Miyazaki T
益生菌有助于增强宿主免疫反应和防止感染。口服益生菌在肠道中诱导 IgA 产生被认为是产生这种有益效果的原因之一，但其作用机制尚不清楚。加氏乳杆菌SBT2055（LG2055）是一种益生菌，具有胆汁耐受性、改善肠道环境的能力，并且具有与腹部肥胖相关的预防作用。在这项研究中，我们发现口服 LG2055 可诱导 IgA 产生，并增加派尔氏淋巴结和小鼠小肠固有层中 IgA+ 细胞群的比率。 LG2055 显着增加了 B 细胞和骨髓源性树突状细胞 (BMDC) 共培养物中 IgA 的量，而 TLR2 信号对此至关重要。此外，还证明 LG2055 刺激 BMDC 促进 TGF-β、BAFF、IL-6 和 IL-10 的产生，这些对于 B 细胞产生 IgA 至关重要。 BAFF 和 LG2055 联合刺激 B 细胞增强了 IgA 产生的诱导。此外，TGF-β信号被证明对于B细胞和BMDC共培养系统中LG2055诱导的IgA产生至关重要，但TGF-β在仅用LG2055刺激的B细胞的培养物中不诱导IgA产生。此外，TGF-β 对于 LG2055 刺激的 BMDC 产生 BAFF、IL-6、IL-10 和 TGF-β 本身至关重要。这些结果表明，TGF-β是由LG2055刺激的BMDC产生的，并且它具有BMDC诱导BAFF、IL-6和IL-10产生所必需的自分泌/旁分泌功能。
Probiotic bacteria provide benefits in enhancing host immune responses and protecting against infection. Induction of IgA production by oral administration of probiotic bacteria in the intestine has been considered to be one reason for this beneficial effect, but the mechanisms of the effect are poorly understood. Lactobacillus gasseri SBT2055 (LG2055) is a probiotic bacterium with properties such as bile tolerance, ability to improve the intestinal environment, and it has preventive effects related to abdominal adiposity. In this study, we have found that oral administration of LG2055 induced IgA production and increased the rate of IgA+ cell population in Peyer's patch and in the lamina propria of the mouse small intestine. The LG2055 markedly increased the amount of IgA in a co-culture of B cells and bone marrow derived dendritic cells (BMDC), and TLR2 signal is critical for it. In addition, it is demonstrated that LG2055 stimulates BMDC to promote the production of TGF-β, BAFF, IL-6, and IL-10, all critical for IgA production from B cells. Combined stimulation of B cells with BAFF and LG2055 enhanced the induction of IgA production. Further, TGF-β signal was shown to be critical for LG2055-induced IgA production in the B cell and BMDC co-culture system, but TGF-β did not induce IgA production in a culture of only B cells stimulated with LG2055. Furthermore, TGF-β was critical for the production of BAFF, IL-6, IL-10, and TGF-β itself from LG2055-stimulated BMDC. These results demonstrate that TGF-β was produced by BMDC stimulated with LG2055 and it has an autocrine/paracrine function essential for BMDC to induce the production of BAFF, IL-6, and IL-10.
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影响因子：
3.7
作者：
Evrard B;Coudeyras S;Dosgilbert A;Charbonnel N;Alamé J;Tridon A;Forestier C
通讯作者：
Forestier C
TACI和BAFF-R介导B细胞中的同种型切换。
影响因子：
15.3
作者：
Castigli, E;Wilson, SA;Scott, S;Dedeoglu, F;Xu, SL;Lam, KP;Bram, RJ;Jabara, H;Geha, RS
通讯作者：
Geha, RS
DOI：
10.1038/nri2335
发表时间：
2008-06
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
56.9
作者：
Fagarasan, S;Muramatsu, M;Honjo, T
通讯作者：
Honjo, T
影响因子：
4.4
作者：
Kanswal, Sunita;Katsenelson, Nora;Akkoyunlu, Mustafa
通讯作者：
Akkoyunlu, Mustafa