Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.
Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.
复制标题
芳香姜黄酮在体外和体内诱导神经干细胞增殖。
DOI：
10.1186/scrt500
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发表时间：
2014-09-26
影响因子：
7.5
通讯作者：
Rueger MA
中科院分区：
文献类型：
--
作者：
Hucklenbroich J;Klein R;Neumaier B;Graf R;Fink GR;Schroeter M;Rueger MA
芳香（ar-）姜黄酮是姜黄的主要生物活性化合物。有人认为，ar-turmerone抑制小胶质细胞的激活，这一特性可能对治疗神经退行性疾病有用。此外，ar-turmerone对神经干细胞（NSCs）的影响仍有待研究。我们将原代胎鼠NSCs暴露于不同浓度的ar-turmerone中。然后观察细胞的增殖和分化潜能。在体内，naïve大鼠接受单次脑室内（i.c.v）注射ar-turmerone。通过无创正电子发射断层扫描（PET）成像和示踪剂[18F]-氟- l -胸腺嘧啶（[18F]FLT）在体内以及在体外评估内源性NSCs的增殖活性。在体外，ar-turmerone增加了培养的NSCs的数量，这是由于增加了NSC的增殖（P < 0.01）。增殖数据由Ki-67 mRNA的qpcr数据支持。在体内和体外，ar-turmerone均能促进NSCs的神经元分化。在体内，灌注ar-turmerone后，成年大鼠心室下区（SVZ）和海马区可动员增殖的NSCs， [18F]FLT-PET和组织学均证实（P < 0.05）。体外和体内数据均表明，ar-turmerone可诱导NSC增殖。因此，Ar-turmerone构成了支持神经系统疾病再生的有希望的候选者。
Aromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated. We exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naïve rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [18F]-fluoro-L-thymidine ([18F]FLT), as well as ex vivo. In vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [18F]FLT-PET and histology (P < 0.05). Both in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease.
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